NHC Key Laboratory of Hormones and Development (Tianjin Medical University), Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University Chu Hsien-I Memorial Hospital, Tianjin Institute of Endocrinology, Tianjin, 300134, China.
NHC Key Laboratory of Hormones and Development (Tianjin Medical University), Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University Chu Hsien-I Memorial Hospital, Tianjin Institute of Endocrinology, Tianjin, 300134, China.
Biochem Biophys Res Commun. 2019 Sep 24;517(3):413-420. doi: 10.1016/j.bbrc.2019.07.114. Epub 2019 Aug 1.
GLUT9 is generally considered to be associated with the uric acid transport, which plays an important role in the regulation of serum uric acid level. In this study, the expression level of miR-143-3p was significantly decreased in hyperuricemia mice model group compared with the normal control by miRNA microarray, the same results were confirmed in the hyperuricemia patients and the healthy control group. It is predicted that GLUT9 may be the target gene of miR-143-3p by target scan and other net-software. GLUT9 as the downstream target gene of miR-143-3p was determinated by fluorescence enzyme activity assay. Western blotting and qRT-PCR indicated that the expression of GLUT9 in human renal tubular epithelial cells transfected with miR-143-3p mimics was significantly reduced. Meanwhile inflammatory factors IL-1β and MCP-1 significantly decreased. In conclusion, miR-143-3p can reduce uric acid reabsorption by inhibiting its downstream target gene GLUT9.
GLUT9 通常被认为与尿酸转运有关，在调节血清尿酸水平方面发挥着重要作用。在这项研究中，通过 miRNA 微阵列发现高尿酸血症小鼠模型组的 miR-143-3p 表达水平明显低于正常对照组，在高尿酸血症患者和健康对照组也得到了同样的结果。通过靶标扫描和其他网络软件预测 GLUT9 可能是 miR-143-3p 的靶基因。荧光酶活性测定确定 GLUT9 是 miR-143-3p 的下游靶基因。Western blotting 和 qRT-PCR 表明，转染 miR-143-3p 模拟物的人肾小管上皮细胞中 GLUT9 的表达明显降低。同时，炎症因子 IL-1β 和 MCP-1 也显著下降。总之，miR-143-3p 通过抑制其下游靶基因 GLUT9 减少尿酸重吸收。
