Pizarro Lorena, Leibman-Markus Meirav, Schuster Silvia, Bar Maya, Avni Adi
School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel.
Department of Plant Pathology and Weed Research, Agricultural Research Organization, Volcani Center, Rishon, Israel.
Front Plant Sci. 2019 Jul 19;10:936. doi: 10.3389/fpls.2019.00936. eCollection 2019.
The endocytic trafficking pathway is employed by the plant to regulate immune responses, and is often targeted by pathogen effectors to promote virulence. The model system of the tomato receptor-like protein (RLP) LeEIX2 and its ligand, the elicitor EIX, employs endocytosis to transmit receptor-mediated signals, with some of the signaling events occurring directly from endosomal compartments. Here, to explore the trafficking mechanism of LeEIX2-mediated immune signaling, we used a proteomic approach to identify LeEIX2-associating proteins. We report the identification of SlDRP2A, a dynamin related protein, as an associating partner for LeEIX2. SlDRP2A localizes at the plasma membrane. Overexpression of SlDRP2A increases the sub-population of LeEIX2 in VHAa1 endosomes, and enhances LeEIX2- and FLS2-mediated defense. The effect of SlDRP2A on induction of plant immunity highlights the importance of endomembrane components and endocytosis in signal propagation during plant immune responses.
植物利用内吞运输途径来调节免疫反应，病原体效应子常常靶向该途径以促进毒力。番茄类受体蛋白（RLP）LeEIX2及其配体激发子EIX的模型系统利用内吞作用来传递受体介导的信号，其中一些信号事件直接发生在内体区室。在此，为了探究LeEIX2介导的免疫信号的运输机制，我们采用蛋白质组学方法来鉴定与LeEIX2相关的蛋白。我们报告鉴定出一种发动蛋白相关蛋白SlDRP2A作为LeEIX2的相关伴侣。SlDRP2A定位于质膜。SlDRP2A的过表达增加了VHAa1内体中LeEIX2的亚群，并增强了LeEIX2和FLS2介导的防御。SlDRP2A对植物免疫诱导的作用突出了内膜成分和内吞作用在植物免疫反应信号传播中的重要性。
