Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Immunopharmacol Immunotoxicol. 2011 Mar;33(1):164-8. doi: 10.3109/08923973.2010.491080. Epub 2010 Jun 10.
The present work aimed to assess the effect of bacterial cellulose (BC) from Acetobacter xylinum on some functions of peritoneal macrophages.
Mice peritoneal macrophages were lavaged and co-cultured with various concentrations of BC in microtiter plate. Viability of macrophages was determined by MTT assay. Nitric oxide (NO) amount was detected in culture supernatants of macrophages by Griess reagent and cytotoxicity effect of culture supernatants on WEHI-164 cells as tumor necrosis factor α (TNF-α) bioassay were done.
MTT assay for stimulated macrophages was not different (p = 0.486) and amount of NO production in all doses was the same as unstimulated control macrophages (p = 0.057). Supernatant of macrophages stimulated with 0.01 mg/mL of BC had cytotoxic effect on WEHI-164 fibrosarcoma cells (p = 0.040).
In this study, we showed that BC had no effect on macrophages viability and amount of NO production. BC suppressed the release of TNF-α from macrophages innate immune cells as indication of beneficial effect on wound healing. This data and further studies would imply use of this biomaterial for more applications.
本研究旨在评估木醋杆菌来源的细菌纤维素（BC）对腹腔巨噬细胞某些功能的影响。
通过腹腔灌洗分离小鼠腹腔巨噬细胞，在微量培养板中与不同浓度的 BC 共培养。通过 MTT 法检测巨噬细胞活力。用 Gries 试剂检测巨噬细胞培养上清液中的一氧化氮（NO）含量，并用 WEHI-164 细胞作为肿瘤坏死因子 α（TNF-α）生物测定检测培养上清液的细胞毒性作用。
刺激巨噬细胞的 MTT 法检测结果无差异（p=0.486），且所有剂量组的 NO 产量与未刺激的对照组巨噬细胞相同（p=0.057）。用 0.01mg/mL BC 刺激的巨噬细胞上清液对 WEHI-164 纤维肉瘤细胞具有细胞毒性作用（p=0.040）。
本研究表明，BC 对巨噬细胞活力和 NO 产量没有影响。BC 抑制了巨噬细胞固有免疫细胞中 TNF-α的释放，表明其对伤口愈合有有益的影响。这些数据和进一步的研究将暗示这种生物材料可用于更多的应用。
