Asadzadeh Azizeh, Seyedhosseini Ghaheh Hooria, Sholehvar Fatemeh, Takhshid Mohammadali, Naghizadeh Mohammad Mehdi
Department of Biology, University of Nourdanesh Institute of Higher Education, Meymeh, Isfahan, Iran.
Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Avicenna J Med Biotechnol. 2019 Jul-Sep;11(3):239-244.
Type 2 Diabetes Mellitus (T2DM) is a serious problem in the world. 5-Hydroxytryptamine (5-HT, serotonin) plays an important role in obesity, glucose control and insulin resistance. The polymorphism of the serotonin transporter gene linked promoter region (5-HTTLPR) might influence 5-HTT expression and serotonin uptake. The polymorphism results in two alleles of L (Long) and S (Short). The aim of the present study was to evaluate the association between 5-HTTLPR genotypes in type 2 diabetes mellitus (T2DM), obesity as well as serum biochemical profiles in Iranian population from 2012 until 2015.
180 patients with T2DM and 180 controls were selected and the frequency of S and L alleles was determined by PCR. Then, the relationship between genotypes, body mass index (BMI) and serum biochemical variables was investigated.
The frequency of S and L alleles in experimental and control groups was the same [for the L allele p=0.754, OR (95%CI)=1.103 (0.597 to 2.041) and for the S allele p=0.906, OR (95%CI)=(0.490 to 1.676)]. However, the mean triglyceride, cholesterol, LDL-C, systolic and diastolic blood pressure levels in the diabetic subjects with LL genotype were significantly higher than LS and SS genotypes (p<0.001) in this population.
The L allele of 5-HTTLPR was related to the increased serum lipids and blood pressure in the diabetic patients. However, there was no relationship between the polymorphism of 5-HTTLPR L/S and T2DM in Iranian population.
2型糖尿病（T2DM）是一个全球性的严重问题。5-羟色胺（5-HT，血清素）在肥胖、血糖控制和胰岛素抵抗中发挥着重要作用。血清素转运体基因连锁启动子区域（5-HTTLPR）的多态性可能影响5-HTT的表达和血清素摄取。这种多态性产生了L（长）和S（短）两个等位基因。本研究的目的是评估2012年至2015年伊朗人群中2型糖尿病（T2DM）、肥胖症患者的5-HTTLPR基因型与血清生化指标之间的关联。
选取180例T2DM患者和180例对照，通过聚合酶链反应（PCR）确定S和L等位基因的频率。然后，研究基因型、体重指数（BMI）与血清生化变量之间的关系。
实验组和对照组中S和L等位基因的频率相同[L等位基因p = 0.754，比值比（95%置信区间）= 1.103（0.597至2.041）；S等位基因p = 0.906，比值比（95%置信区间）=（0.490至1.676）]。然而，在该人群中，LL基因型糖尿病患者的甘油三酯、胆固醇、低密度脂蛋白胆固醇、收缩压和舒张压水平显著高于LS和SS基因型患者（p < 0.001）。
5-HTTLPR的L等位基因与糖尿病患者血清脂质和血压升高有关。然而，在伊朗人群中，5-HTTLPR L/S多态性与T2DM之间没有关联。
