Ozbek Emin, Tasci Ali I, Tugcu Volkan, Ilbey Yusuf O, Simsek Abdulmuttalip, Ozcan Levent, Polat Emre C, Koksal Vedat
Department of Urology, Bezm-i Alem Valide Sultan Vakif Gureba Research and Education Hospital, Istanbul 34095, Turkey.
Asian J Androl. 2009 May;11(3):351-5. doi: 10.1038/aja.2008.3. Epub 2009 Mar 2.
We evaluated the genotypes of the serotonin transporter gene (5-HTT) in patients with premature ejaculation (PE) to determine the role of genetic factors in the etiopathogenesis of PE and possibly to identify the patient subgroups. A total of 70 PE patients and 70 controls were included in this study. All men were heterosexual, had no other disorders and were either married or in a stable relationship. PE was defined as ejaculation that occurred within 1 min of vaginal intromission. Genomic DNA from patients and controls was analyzed using polymerase chain reaction, and allelic variations of the promoter region of the serotonin transporter gene (5-HTTLPR) were determined. The 5-HTTLPR (serotonin transporter promoter gene) genotypes in PE patients vs. controls were distributed as follows: L/L 16% vs. 17%, L/S 30% vs. 53% and S/S 54% vs. 28%. We examined the haplotype analysis for three polymorphisms of the 5-HTTLPR gene: LL, LS and SS. The appropriateness of the allele frequencies in the 5-HTTLPR gene was analyzed by the Hardy-Weinberg equilibrium using the chi2-test. The short (S) allele of the 5-HTTLPR gene was significantly more frequent in PE patients than in controls (P<0.05). We suggest that the 5-HTTLPR gene plays a role in the pathophysiology of all primary PE cases. Further studies are needed to evaluate the relationship between 5-HTTLPR gene polymorphism and patient subgroup (such as primary and secondary PE) responses to selective serotonin reuptake inhibitors as well as ethnic differences.
我们评估了早泄（PE）患者血清素转运体基因（5-HTT）的基因型，以确定遗传因素在PE发病机制中的作用，并可能识别患者亚组。本研究共纳入70例PE患者和70例对照。所有男性均为异性恋，无其他疾病，已婚或处于稳定关系。PE定义为阴道插入后1分钟内射精。使用聚合酶链反应分析患者和对照的基因组DNA，并确定血清素转运体基因启动子区域（5-HTTLPR）的等位基因变异。PE患者与对照的5-HTTLPR（血清素转运体启动子基因）基因型分布如下：L/L型分别为16%和17%，L/S型分别为30%和53%，S/S型分别为54%和28%。我们对5-HTTLPR基因的三种多态性进行了单倍型分析：LL、LS和SS。使用卡方检验通过哈迪-温伯格平衡分析5-HTTLPR基因中等位基因频率的适宜性。5-HTTLPR基因的短（S）等位基因在PE患者中比在对照中显著更常见（P<0.05）。我们认为5-HTTLPR基因在所有原发性PE病例的病理生理学中起作用。需要进一步研究来评估5-HTTLPR基因多态性与患者亚组（如原发性和继发性PE）对选择性5-羟色胺再摄取抑制剂的反应以及种族差异之间的关系。
