• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种转录适体传感器：通过点亮 RNA 适体实现食源性病原体的放大、无标记和无需培养的检测。

A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens via light-up RNA aptamers.

机构信息

College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China.

出版信息

Chem Commun (Camb). 2019 Aug 20;55(68):10096-10099. doi: 10.1039/c9cc05036a.

DOI:10.1039/c9cc05036a
PMID:31380872
Abstract

We report a transcription aptasensor by using a light-up RNA aptamer. It allows for sensitive, label-free and culture-free detection of intact foodborne pathogens, and no separation, purification or enrichment processes are involved.

摘要

我们报道了一种使用点亮 RNA 适体的转录适体传感器。它可以实现对完整食源性病原体的灵敏、无标记和无培养检测，并且不涉及分离、纯化或富集过程。

相似文献

1
A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens via light-up RNA aptamers.一种转录适体传感器：通过点亮 RNA 适体实现食源性病原体的放大、无标记和无需培养的检测。
Chem Commun (Camb). 2019 Aug 20;55(68):10096-10099. doi: 10.1039/c9cc05036a.
2
A fluorescent aptasensor for Staphylococcus aureus based on strand displacement amplification and self-assembled DNA hexagonal structure.基于链置换扩增和自组装 DNA 六方结构的金黄色葡萄球菌荧光适体传感器。
Mikrochim Acta. 2020 Apr 30;187(5):304. doi: 10.1007/s00604-020-04293-9.
3
Functional chimera aptamer and molecular beacon based fluorescent detection of Staphylococcus aureus with strand displacement-target recycling amplification.基于功能嵌合体适体和分子信标的荧光检测金黄色葡萄球菌的链置换靶标循环扩增。
Anal Chim Acta. 2019 Oct 10;1075:128-136. doi: 10.1016/j.aca.2019.05.014. Epub 2019 May 8.
4
Light-up RNA aptamer enabled label-free protein detection via a proximity induced transcription assay.基于近邻诱导转录检测的亮报告 RNA 适体无标记蛋白检测方法
Chem Commun (Camb). 2018 Aug 7;54(64):8877-8880. doi: 10.1039/c8cc04498h.
5
Ultrasensitive Fluorometric Angling Determination of in Vitro and Fluorescence Imaging in Vivo Using Carbon Dots with Full-Color Emission.基于全彩色发射碳点的超灵敏荧光角度测定法用于体外和体内的荧光成像。
Anal Chem. 2019 Nov 19;91(22):14681-14690. doi: 10.1021/acs.analchem.9b03916. Epub 2019 Oct 28.
6
G-quadruplex specific thioflavin T-based label-free fluorescence aptasensor for rapid detection of tetracycline.基于硫堇 T 的 G-四链体特异性无标记荧光适体传感器快速检测四环素。
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Sep 5;238:118406. doi: 10.1016/j.saa.2020.118406. Epub 2020 Apr 23.
7
Graphene-based label-free electrochemical aptasensor for rapid and sensitive detection of foodborne pathogen.基于石墨烯的无标记电化学适体传感器用于快速灵敏检测食源性病原体。
Anal Bioanal Chem. 2017 Nov;409(29):6893-6905. doi: 10.1007/s00216-017-0654-6. Epub 2017 Oct 13.
8
SiC-functionalized fluorescent aptasensor for determination of Proteus mirabilis.碳化硅功能化荧光适体传感器用于检测奇异变形杆菌。
Mikrochim Acta. 2020 Jun 27;187(7):406. doi: 10.1007/s00604-020-04378-5.
9
Directly profiling intact Staphylococcus aureus in water and foods via enzymatic cleavage aptasensor.通过酶切适体传感器直接分析水和食品中的完整金黄色葡萄球菌。
Anal Chim Acta. 2020 Oct 2;1132:28-35. doi: 10.1016/j.aca.2020.07.058. Epub 2020 Aug 6.
10
Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1.抗金黄色葡萄球菌肠毒素C1的DNA适配体的筛选与鉴定
Food Chem. 2015 Jan 1;166:623-629. doi: 10.1016/j.foodchem.2014.06.039. Epub 2014 Jun 21.

引用本文的文献

1
Molecular methods for rapid detection and identification of foodborne pathogenic bacteria.用于快速检测和鉴定食源性病原体细菌的分子方法。
World J Microbiol Biotechnol. 2025 May 15;41(5):175. doi: 10.1007/s11274-025-04396-6.
2
An ultrasensitive label-free RNase H assay based on transcription of fluorogenic light-up aptamer.一种基于荧光点亮适体转录的超灵敏无标记核糖核酸酶H检测方法。
Nanoscale Adv. 2024 Mar 7;6(7):1926-1931. doi: 10.1039/d3na00975k. eCollection 2024 Mar 26.
3
Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review).
分子诊断技术在检测传染病病原体中的应用进展（综述）。
Mol Med Rep. 2023 May;27(5). doi: 10.3892/mmr.2023.12991. Epub 2023 Apr 7.
4
Recent Advances in Aptasensors For Rapid and Sensitive Detection of .用于快速灵敏检测的适体传感器的最新进展
Front Bioeng Biotechnol. 2022 May 23;10:889431. doi: 10.3389/fbioe.2022.889431. eCollection 2022.
5
Biosensor Technologies in Medicine: from Detection of Biochemical Markers to Research into Molecular Targets (Review).生物传感器技术在医学中的应用：从生化标志物检测到分子靶标研究（综述）。
Sovrem Tekhnologii Med. 2021;12(6):70-83. doi: 10.17691/stm2020.12.6.09. Epub 2020 Dec 28.
6
Oligonucleotide aptamers for pathogen detection and infectious disease control.寡核苷酸适体在病原体检测和传染病控制中的应用。
Theranostics. 2021 Aug 27;11(18):9133-9161. doi: 10.7150/thno.61804. eCollection 2021.
7
Three-Way Junction-Induced Isothermal Amplification with High Signal-to-Background Ratio for Detection of Pathogenic Bacteria.三向结引发的高信号背景比等温扩增用于致病性细菌的检测。
Sensors (Basel). 2021 Jun 16;21(12):4132. doi: 10.3390/s21124132.
8
Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction.基于双生物素-链霉亲和素相互作用的竞争性 HRP 偶联比色适体传感器用于检测食品中的伏马菌素 B1。
Biosensors (Basel). 2020 Mar 30;10(4):31. doi: 10.3390/bios10040031.
9
Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors.将适体和核酸酶拆分用于开发先进的生物传感器。
Nucleic Acids Res. 2020 Apr 17;48(7):3400-3422. doi: 10.1093/nar/gkaa132.
10
Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends.无标记生物传感器在基于实验室的传染病诊断中的应用：当前的成就和新趋势。
Biosensors (Basel). 2020 Feb 12;10(2):11. doi: 10.3390/bios10020011.