Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Phytochem Anal. 2020 Mar;31(2):191-203. doi: 10.1002/pca.2880. Epub 2019 Aug 5.
Natural products are obtaining much acceptance as ergogenic aid, not only among athletes but also among the general population including people with excess body fat. Under normal circumstances, an obese person will have the desire and ability to exercise reduced; mainly because they are easily fatigued. Thus, they need to boost their energy production so that they can be more active and healthier.
In this present work, Morinda citrifolia L. leaf extract (MLE) which is believed to possess ergogenic property, was evaluated on its effect on an obese animal model using H-NMR based metabolomics.
Rats were fed with high fat diet (HFD) for 12 weeks for obese development. Once this was achieved, all the rats underwent endurance exercise (forced swimming test) every 2 weeks for 8 weeks together with treatment. The time to exhaustion was recorded for each rat. Three different dosages of MLE: 50 mg/kg, 100 mg/kg and 200 mg/kg of body weight were used together with two positive controls: 5 mg/kg caffeine and 100 mg/kg green tea. Blood was collected before and after treatments for metabolomics study.
Findings showed that feeding the rats at a dose of 200 mg/kg body weight MLE significantly prolonged the exhaustive swimming time of the rats, and altered the metabolites present in their serum. Discriminating metabolites involved were the product of various metabolic pathways, including carbohydrate, lipids metabolism and energy metabolism. Treatment with 200 mg/kg body weight MLE resulted in significant improvement in the metabolic perturbations where the proximity of the obese exercised treated group to that of normal exercised group in the partial least squares discriminant analysis score plot was observed.
The present work demonstrated ergogenic property of MLE based on the improved metabolic perturbation in exercised obese rats.
天然产物作为一种运动补剂越来越受到欢迎，不仅在运动员中，在普通人群中，包括超重人群中也是如此。在正常情况下，肥胖者的运动欲望和能力会降低；主要是因为他们容易疲劳。因此，他们需要提高能量产生，以便更活跃和更健康。
在本研究中，我们评估了被认为具有运动性能的桃金娘科叶提取物（MLE）对肥胖动物模型的影响，使用基于 H-NMR 的代谢组学方法。
将大鼠用高脂肪饮食（HFD）喂养 12 周，以促进肥胖的发展。一旦达到肥胖状态，所有大鼠每隔 2 周进行一次耐力运动（强迫游泳试验），同时进行治疗。记录每只大鼠的力竭时间。使用了三种不同剂量的 MLE：50mg/kg、100mg/kg 和 200mg/kg 体重，以及两种阳性对照物：5mg/kg 咖啡因和 100mg/kg 绿茶。在治疗前后采集血液进行代谢组学研究。
结果表明，以 200mg/kg 体重剂量给大鼠喂食 MLE 显著延长了大鼠的游泳时间，并改变了它们血清中的代谢物。涉及的差异代谢物是多种代谢途径的产物，包括碳水化合物、脂质代谢和能量代谢。用 200mg/kg 体重 MLE 治疗，显著改善了代谢紊乱，肥胖运动治疗组与正常运动组在偏最小二乘判别分析得分图中的接近度表明了这一点。
本研究证明了 MLE 的运动性能，基于运动肥胖大鼠代谢紊乱的改善。
