Butsayawarapat Pimprapai, Juntawong Piyada, Khamsuk Ornusa, Somta Prakit
Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Bangkok 10900, Thailand.
Plants (Basel). 2019 Aug 2;8(8):264. doi: 10.3390/plants8080264.
(zombi pea) is an underutilized legume crop considered to be a potential gene source in breeding for abiotic stress tolerance. This study focuses on the molecular characterization of mechanisms controlling waterlogging tolerance using two zombi pea varieties with contrasting waterlogging tolerance. Morphological examination revealed that in contrast to the sensitive variety, the tolerant variety was able to grow, maintain chlorophyll, form lateral roots, and develop aerenchyma in hypocotyl and taproots under waterlogging. To find the mechanism controlling waterlogging tolerance in zombi pea, comparative transcriptome analysis was performed using roots subjected to short-term waterlogging. Functional analysis indicated that glycolysis and fermentative genes were strongly upregulated in the sensitive variety, but not in the tolerant one. In contrast, the genes involved in auxin-regulated lateral root initiation and formation were expressed only in the tolerant variety. In addition, cell wall modification, aquaporin, and peroxidase genes were highly induced in the tolerant variety under waterlogging. Our findings suggest that energy management and root plasticity play important roles in mitigating the impact of waterlogging in zombi pea. The basic knowledge obtained from this study can be used in the molecular breeding of waterlogging-tolerant legume crops in the future.
（僵尸豌豆）是一种未得到充分利用的豆科作物，被认为是培育非生物胁迫耐受性的潜在基因来源。本研究聚焦于利用两个耐涝性不同的僵尸豌豆品种，对控制耐涝性的机制进行分子特征分析。形态学检查表明，与敏感品种相比，耐涝品种在涝渍条件下能够生长、维持叶绿素含量、形成侧根，并在胚轴和主根中发育通气组织。为了找出僵尸豌豆耐涝性的控制机制，对遭受短期涝渍的根部进行了比较转录组分析。功能分析表明，糖酵解和发酵基因在敏感品种中强烈上调，但在耐涝品种中没有。相反，参与生长素调节侧根起始和形成的基因仅在耐涝品种中表达。此外，细胞壁修饰、水通道蛋白和过氧化物酶基因在耐涝品种受涝渍时高度诱导。我们的研究结果表明，能量管理和根可塑性在减轻僵尸豌豆涝渍影响方面发挥着重要作用。本研究获得的基础知识可用于未来耐涝豆科作物的分子育种。
