Sreeratree Jaruwan, Butsayawarapat Pimprapai, Chaisan Tanapon, Somta Prakit, Juntawong Piyada
Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
Plants (Basel). 2022 Mar 30;11(7):930. doi: 10.3390/plants11070930.
Global climate changes increase the frequency and intensity of heavy precipitation events, which result in flooding or soil waterlogging. One way to overcome these low-oxygen stresses is via modifying the plant root system to improve internal aeration. Here, we used a comparative RNA-seq based transcriptomic approach to elucidate the molecular mechanisms of waterlogging-triggered root plasticity in mungbean (), a major grain legume cultivated in Asia. Two mungbean varieties with contrasting waterlogging tolerance due to the plasticity of the root system architecture were subjected to short-term and long-term waterlogging. Then, RNA-seq was performed. Genes highly expressed in both genotypes under short-term waterlogging are related to glycolysis and fermentation. Under long-term waterlogging, the expression of these genes was less induced in the tolerant variety, suggesting it had effectively adapted to waterlogging via enhancing root plasticity. Remarkably, under short-term waterlogging, the expression of several transcription factors that serve as integrators for ethylene and jasmonic acid signals controlling root stem cell development was highly upregulated only in the tolerant variety. Sequentially, root development-related genes were more expressed in the tolerant variety under long-term waterlogging. Our findings suggest that ethylene and jasmonic acids may contribute to waterlogging-triggered root plasticity by relaying environmental signals to reprogram root regeneration. This research provides the basis for the breeding and genetic engineering of waterlogging-tolerant crops in the future.
全球气候变化增加了强降水事件的频率和强度，导致洪水或土壤渍水。克服这些低氧胁迫的一种方法是通过改良植物根系来改善内部通气。在此，我们采用基于比较RNA测序的转录组学方法，以阐明亚洲主要种植的豆类作物绿豆中渍水引发的根系可塑性的分子机制。两个因根系结构可塑性而具有不同耐渍性的绿豆品种分别遭受短期和长期渍水。然后，进行RNA测序。在短期渍水条件下，两个基因型中高表达的基因与糖酵解和发酵有关。在长期渍水条件下，这些基因在耐渍品种中的诱导表达较少，表明其通过增强根系可塑性有效适应了渍水。值得注意的是，在短期渍水条件下，几个作为控制根干细胞发育的乙烯和茉莉酸信号整合因子的转录因子的表达仅在耐渍品种中高度上调。随后，在长期渍水条件下，与根系发育相关的基因在耐渍品种中表达更多。我们的研究结果表明，乙烯和茉莉酸可能通过传递环境信号来重新编程根系再生，从而促进渍水引发的根系可塑性。这项研究为未来耐渍作物的育种和基因工程提供了基础。
