使用单克隆抗体的四分子复合物将T细胞免疫吸附到玻璃珠上。

Immunoadsorption of T cells onto glass beads using tetramolecular complexes of monoclonal antibodies.

作者信息

Thomas T E, Lansdorp P M

机构信息

Terry Fox Laboratory, Cancer Control Agency of B.C., Vancouver, Canada.

出版信息

J Immunol Methods. 1988 Sep 13;112(2):219-26. doi: 10.1016/0022-1759(88)90361-4.

DOI:10.1016/0022-1759(88)90361-4

PMID:3138310

Abstract

Tetramolecular monoclonal antibody complexes were used to selectively cross-link a subset of human peripheral blood T cells (CD8 positive) to glass beads coated with hapten (fluorescein) modified bovine serum albumin. Tetramolecular antibody complexes were prepared with anti-CD8 (Leu 2a), anti-FITC mouse IgG1 monoclonal antibodies and monoclonal rat anti-mouse IgG1. Optimum conditions for depletion of CD8 positive cells from peripheral blood mononuclear cell suspensions were determined with 1 ml columns. 90-99% of the CD8 positive cells could be removed with 0-17% non-specific adsorption of CD8 negative cells. The weakest link in this system was the bond between hapten-modified albumin and the glass beads. These results indicate that tetramolecular antibody complexes are useful for the specific immunoadsorption of cells to a defined affinity matrix.

摘要

四分子单克隆抗体复合物被用于将一部分人外周血T细胞（CD8阳性）选择性地交联到包被有半抗原（荧光素）修饰牛血清白蛋白的玻璃珠上。用抗CD8（Leu 2a）、抗荧光素异硫氰酸酯小鼠IgG1单克隆抗体和大鼠抗小鼠IgG1单克隆抗体制备四分子抗体复合物。用1 ml柱确定从外周血单核细胞悬液中去除CD8阳性细胞的最佳条件。90%-99%的CD8阳性细胞能够被去除，同时CD8阴性细胞的非特异性吸附率为0%-17%。该系统中最薄弱的环节是半抗原修饰白蛋白与玻璃珠之间的结合。这些结果表明，四分子抗体复合物可用于将细胞特异性免疫吸附到特定的亲和基质上。