Institute of Macromolecules (IMA/UFRJ), Federal University of Rio de Janeiro, Av. Horácio Macedo, 2030, 21941598 RJ, Brazil.
J Nanosci Nanotechnol. 2020 Jan 1;20(1):31-41. doi: 10.1166/jnn.2020.16875.
Consumer demand for cosmetics is growing, causing a need to develop new systems to release active ingredients. Among these, nanostructured lipid carriers (NLCs) have certain advantages regarding penetration of active compounds in the skin. The study reported here aimed to develop an NLC system for controlled release of vitamin E, a substance that has antioxidant and photoprotective properties. The NLCs containing vitamin E (NLC-VE) were prepared by the melting-emulsionsolidification method, using beeswax as the solid lipid, medium-chain triglycerides (MCTs), coconut oil or avocado oil as liquid lipids and three different nonionic surfactants. The composition of the system was defined by studying the effect of various experimental factors on the size distribution, average diameter and physical stability of the nanoparticles. The optimization of these characteristics, achieved by a Box-Behnken statistical design, showed that 8% w/w of the nonionic surfactant Tween 80, 24% ultrasound amplitude and processing time of 2 minutes and 16 seconds generated nanoparticles with homogeneous size (PDI = 0.11±0.02), average diameter of 180±20 nm and physical stability of 12 weeks. The NLC-VE systems prepared under the optimal conditions, containing Tween 80, beeswax and MCTs, were formulated as viscous suspensions by adding Pluronic F-127, a poly(ethylene oxide)-poly(propylene oxide) block copolymer, at a concentration of 10% w/w. The colloidal nanosuspension obtained had a viscosity of 222 mPa·s and released 70% of the active substance in 6 hours, indicating it is a promising candidate for controlled release of vitamin E.
消费者对化妆品的需求不断增长，这促使人们需要开发新的系统来释放活性成分。在这些系统中，纳米结构脂质载体（NLC）在活性化合物渗透皮肤方面具有一定优势。本研究旨在开发一种用于控制释放维生素 E 的 NLC 系统，维生素 E 是一种具有抗氧化和光保护特性的物质。通过熔融乳化-固化法制备了含有维生素 E 的 NLC（NLC-VE），其中蜂蜡为固体脂质，中链甘油三酯（MCT）、椰子油或鳄梨油为液体脂质，三种不同的非离子表面活性剂。通过研究各种实验因素对纳米粒子的粒径分布、平均直径和物理稳定性的影响，确定了系统的组成。通过 Box-Behnken 统计设计对这些特性进行优化，结果表明，8%w/w 的非离子表面活性剂 Tween 80、24%超声幅度和 2 分钟 16 秒的处理时间产生了粒径均匀（PDI=0.11±0.02）、平均直径为 180±20nm 且物理稳定性为 12 周的纳米粒子。在最佳条件下制备的 NLC-VE 系统，含有 Tween 80、蜂蜡和 MCT，通过添加聚氧乙烯-聚氧丙烯嵌段共聚物 Pluronic F-127（浓度为 10%w/w）配制成粘性混悬剂。所得胶体纳米混悬液的粘度为 222mPa·s，在 6 小时内释放了 70%的活性物质，表明其是维生素 E 控制释放的有前途的候选物。
