Bryson Alexandra L, Hill Emily M, Doern Christopher D
Department of Pathology, Virginia Commonwealth University Health System, 403 North 13th Street, Richmond, VA 23298, USA.
Pathology & Laboratory Medicine, Hunter Holmes McGuire VA Medical Center, 1201 Broad Rock Boulevard, Richmond, VA 23224, USA.
Clin Lab Med. 2019 Sep;39(3):391-404. doi: 10.1016/j.cll.2019.05.010. Epub 2019 Jul 2.
This article summarizes recent advances in the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to new areas of infectious diseases diagnostics. We discuss progress toward routine identification of mycobacteria and filamentous fungi and direct identification of pathogens from clinical specimens. Of greatest interest is the use of MALDI-TOF MS for identifying organisms from positive blood cultures and from clinical specimens such as urine. Last, We highlight some exciting new possibilities for MALDI-TOF MS phenotypic susceptibility testing for bacteria and yeast.
本文总结了基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）在传染病诊断新领域应用的最新进展。我们讨论了在分枝杆菌和丝状真菌常规鉴定以及从临床标本中直接鉴定病原体方面取得的进展。最令人感兴趣的是使用MALDI-TOF MS从阳性血培养物和尿液等临床标本中鉴定微生物。最后，我们强调了MALDI-TOF MS用于细菌和酵母表型药敏试验的一些令人兴奋的新可能性。
