使用基质辅助激光解吸电离飞行时间质谱法鉴定口腔鳞状细胞癌患者的需氧唾液微生物：一项初步研究的初步结果
Identification of Aerobic Salivary Microorganisms in Patients With Oral Squamous Cell Carcinoma Using MALDI-TOF MS: Preliminary Findings From a Pilot Study.
作者信息
da Silva Moreira Milena, Schmitz Giovanna, de Sá Alves Mariana, Mendes Maria Anita, Alves Mônica Ghislaine Oliveira, Alves Levy Anderson César, Dias Meriellen, Bandeira Celso Muller, Nepomuceno Gabrielle Luana Jimenez Teodoro, da Silva Martinho Herculano, Almeida Janete Dias
机构信息
Universidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, Campus São José dos Campos, São José dos Campos, São Paulo, Brazil.
Dempster MS Lab, Universidade de São Paulo, São Paulo, Brazil.
出版信息
Rapid Commun Mass Spectrom. 2025 Aug 15;39(15):e10063. doi: 10.1002/rcm.10063.
RATIONALE
Oral squamous cell carcinoma (OSCC) is a multifactorial disease, and emerging evidence links the oral microbiome to its development. Rapid, noninvasive identification of salivary microorganisms may offer novel diagnostic and prognostic insights into oral cancer.
METHODS
Salivary microbiota from OSCC patients and healthy individuals were analyzed using MALDI Biotyper. Saliva samples were cultured, and microbial identification was performed based on protein spectral profiles using an UltrafleXtreme MALDI-TOF mass spectrometer.
RESULTS
Thirteen OSCC patients (mean age 55 ± 11 years; 69% male) and nineteen healthy controls (mean age 55 ± 10 years; 79% male) were analyzed. Distinct microbial profiles were observed in OSCC patients, including pathogenic species previously associated with carcinogenesis, suggesting potential biomarkers for oral cancer.
CONCLUSIONS
The MALDI Biotyper is an effective, noninvasive tool for identifying salivary microbiota. Its application may support early diagnosis and prognosis of OSCC, reinforcing the significance of the oral microbiome in cancer etiology.
原理
口腔鳞状细胞癌（OSCC）是一种多因素疾病，越来越多的证据将口腔微生物群与其发展联系起来。快速、无创地识别唾液微生物可能为口腔癌提供新的诊断和预后见解。
方法
使用基质辅助激光解吸电离飞行时间质谱仪（MALDI Biotyper）分析OSCC患者和健康个体的唾液微生物群。对唾液样本进行培养，并使用UltrafleXtreme MALDI-TOF质谱仪基于蛋白质光谱图谱进行微生物鉴定。
结果
分析了13例OSCC患者（平均年龄55±11岁；69%为男性）和19名健康对照者（平均年龄55±10岁；79%为男性）。在OSCC患者中观察到不同的微生物谱，包括先前与致癌作用相关的致病物种，提示可能为口腔癌的生物标志物。
结论
MALDI Biotyper是一种用于识别唾液微生物群的有效、无创工具。其应用可能有助于OSCC的早期诊断和预后，强化口腔微生物群在癌症病因学中的重要性。
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