Mitchell Stephanie L, Simner Patricia J
Department of Pathology, UPMC Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Avenue, Main Hospital, Floor B, #269, Pittsburgh, PA 15224, USA.
Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Meyer B1-193, 600 North Wolfe Street, Baltimore, MD 21287-7093, USA.
Clin Lab Med. 2019 Sep;39(3):405-418. doi: 10.1016/j.cll.2019.05.003.
Next-generation sequencing (NGS) applications have been transitioning from research tools to diagnostic methods and are becoming more commonplace in clinical microbiology laboratories. These applications include (1) whole-genome sequencing, (2) targeted next-generation sequencing methods, and (3) metagenomic next-generation sequencing. The introduction of these methods into the clinical microbiology laboratory has led to the theoretic question of "Will NGS-based methods supplant traditional methods for strain typing, identification, and antimicrobial susceptibility prediction?" The authors address this question and discuss where we are at now with clinical NGS applications for infectious diseases, what does the future hold, and at what cost?
下一代测序（NGS）技术已从研究工具转变为诊断方法，在临床微生物学实验室中越来越普遍。这些应用包括：（1）全基因组测序；（2）靶向下一代测序方法；（3）宏基因组下一代测序。将这些方法引入临床微生物学实验室引发了一个理论问题：“基于NGS的方法会取代传统的菌株分型、鉴定及抗菌药物敏感性预测方法吗？”作者探讨了这一问题，并讨论了目前传染病临床NGS应用的现状、未来趋势以及成本如何。
