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现状评估：传染病诊断的新一代测序技术。

Status check: next-generation sequencing for infectious-disease diagnostics.

机构信息

Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Department of Pathology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.

出版信息

J Clin Invest. 2024 Feb 15;134(4):e178003. doi: 10.1172/JCI178003.

DOI:10.1172/JCI178003
PMID:38357923
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10866643/
Abstract

Next-generation sequencing (NGS) applications for the diagnostics of infectious diseases has demonstrated great potential with three distinct approaches: whole-genome sequencing (WGS), targeted NGS (tNGS), and metagenomic NGS (mNGS, also known as clinical metagenomics). These approaches provide several advantages over traditional microbiologic methods, though challenges still exist.

摘要

下一代测序 (NGS) 在传染病诊断方面的应用具有巨大的潜力，主要有三种不同的方法：全基因组测序 (WGS)、靶向 NGS (tNGS) 和宏基因组 NGS (mNGS，也称为临床宏基因组学)。与传统的微生物学方法相比，这些方法具有许多优势，但仍然存在挑战。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/757e/10866643/429a5274aac9/jci-134-178003-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/757e/10866643/429a5274aac9/jci-134-178003-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/757e/10866643/429a5274aac9/jci-134-178003-g001.jpg

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