National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia; Far Eastern Federal University, Vladivostok, 690950, Russia.
National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia.
Tissue Cell. 2019 Aug;59:1-9. doi: 10.1016/j.tice.2019.05.006. Epub 2019 May 22.
The effect of the GM6001 metalloproteinase inhibitor on the regeneration of ambulacral structures in Eupentacta fraudatrix has been investigated. Inhibition of proteinase activity exerts a marked effect on regeneration, being dependent on the time when GM6001 is injected. When administration of the inhibitor begins on day 3 post-injury, regeneration is completely abolished, and the animals die. This means that early activation of proteinases is crucial for triggering the regenerative process in holothurians. When GM6001 in first injected on day 7 post-injury, the regeneration rate decreases. However, this effect has proven to be reversible: when inhibition ceases, the regeneration resumes. The effect of the inhibitor is manifested as a retarded degradation of the extracellular matrix, the lack of cell dedifferentiation, and, probably, a slower cell migration. The gelatinase activity is detected in all the regenerating organs of E. fraudatrix. In the holothurian Cucumaria japonica, which is not capable of healing skin wounds and ambulacrum reparation, no gelatinase activity was observed at the site of damage. A suggestion is made that proteinases play an important role in regeneration in holothurians. The most probable morphogenesis regulators are matrix metalloproteinases with gelatinase activity.
GM6001 金属蛋白酶抑制剂对 Eupentacta fraudatrix 步带结构再生的影响。研究了蛋白酶活性的抑制对再生的显著影响，这种影响依赖于 GM6001 注射的时间。当抑制剂在损伤后第 3 天开始给药时，再生完全被抑制，动物死亡。这意味着早期激活蛋白酶对于触发海参的再生过程至关重要。当 GM6001 在损伤后第 7 天首次注射时，再生速度降低。然而，这种效果是可逆的：当抑制停止时，再生恢复。抑制剂的作用表现为细胞外基质降解延迟，细胞去分化缺失，并且可能细胞迁移速度较慢。在 E. fraudatrix 的所有再生器官中均检测到明胶酶活性。在不能治愈皮肤伤口和修复步带的海参 Cucumaria japonica 中，在损伤部位未观察到明胶酶活性。提出了蛋白酶在海参再生中发挥重要作用的观点。最有可能的形态发生调节剂是具有明胶酶活性的基质金属蛋白酶。
