Zama Uyrá, Lino-Neto José, Dolder Heidi
Departamento de Biologia Celular, Instituto de Biologia, CP 6109-UNICAMP, CEP: 13084-971, Campinas, São Paulo, Brazil.
Tissue Cell. 2004 Feb;36(1):29-41. doi: 10.1016/j.tice.2003.08.003.
In spite of their importance in the reproduction of phanerograms, few bees have received an adequate description of their sperm ultrastructure. In this study, we concluded that it was possible to define a characteristic pattern for Meliponini spermatozoa. However, we have also found species-specific differences that could be used, for example, in a taxonomic or phylogenetic evaluation. Meliponini spermatozoa are made up of a head and a flagellar region. The head includes an acrosome containing the perforatorium, covered by the acrosomal vesicle and a nucleus. In transverse sections, the acrosome is circular at the tip but becomes triangular as it nears the nucleus. The perforatorium base penetrates into a small cavity in the nuclear tip. The flagellum consists of an axoneme, a pair of mitochondrial derivatives, a centriolar adjunct and a pair of accessory bodies. The axoneme has a typical 9+9+2 microtubule pattern. In the final portion, the axoneme becomes gradually disorganized with the accessory microtubules terminating last. The mitochondrial derivatives are asymmetric in length and diameter. In cross-section, they are ellipsoidal and the larger one has a paracrystalline region. The centriolar adjunct begins at the nuclear base and extends parallel to the axoneme up to the anterior end of the smaller mitochondrial derivative.
尽管蜜蜂在显花植物繁殖中具有重要作用，但很少有蜜蜂的精子超微结构得到充分描述。在本研究中，我们得出结论，有可能确定无刺蜂精子的特征模式。然而，我们也发现了物种特异性差异，例如可用于分类学或系统发育评估。无刺蜂精子由头部和鞭毛区组成。头部包括一个含有穿孔器的顶体，顶体泡覆盖着顶体，还有一个细胞核。在横切面上，顶体尖端呈圆形，但靠近细胞核时变为三角形。穿孔器基部插入核尖端的一个小腔中。鞭毛由轴丝、一对线粒体衍生物、一个中心粒附属物和一对辅助体组成。轴丝具有典型的9+9+2微管模式。在最后部分，轴丝逐渐紊乱，辅助微管最后终止。线粒体衍生物在长度和直径上不对称。在横切面上，它们呈椭圆形，较大的一个有一个平行排列的区域。中心粒附属物始于核基部，与轴丝平行延伸至较小线粒体衍生物的前端。
