基于iTRAQ技术的血浆蛋白质组学分析寻找2型早期糖尿病肾病的候选生物标志物
iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.
作者信息
Lu Hongmei, Deng Shaodong, Zheng Minghui, Hu Kunhua
机构信息
1The Second Clinical Medical College, Guangdong Medical University, Dongguan, 523808 China.
2Department of Clinical Laboratory, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, 510120 China.
出版信息
Clin Proteomics. 2019 Jul 31;16:33. doi: 10.1186/s12014-019-9253-1. eCollection 2019.
BACKGROUND
Diabetic nephropathy is the most frequent cause of end-stage renal disease worldwide. Identification of biomarkers for diabetic nephropathy for early diagnosis may be the key to avoiding damage from this condition.
METHODS
Proteomic iTRAQ technology was first used to identify differentially expressed plasma proteins in type 2 incipient diabetic nephropathy (IDN) using a Q-Exactive mass spectrometer.
RESULTS
Compared with controls, 57 proteins (32 upregulated and 25 downregulated proteins) were identified. Furthermore, the gelsolin, collectin-11, PTPRJ, and AKAP-7 proteins were confirmed by Western blots as candidate biomarkers for type 2 IDN through ROC analysis.
CONCLUSIONS
These findings offer a theoretical basis for the early treatment of diabetic nephropathy.
背景
糖尿病肾病是全球终末期肾病最常见的病因。识别用于早期诊断糖尿病肾病的生物标志物可能是避免该疾病造成损害的关键。
方法
首次使用蛋白质组iTRAQ技术，通过Q-Exactive质谱仪鉴定2型早期糖尿病肾病（IDN）中差异表达的血浆蛋白。
结果
与对照组相比，鉴定出57种蛋白质（32种上调蛋白和25种下调蛋白）。此外，通过蛋白质免疫印迹法证实凝溶胶蛋白、凝集素-11、蛋白酪氨酸磷酸酶受体J和A激酶锚定蛋白7为2型IDN的候选生物标志物，并进行了ROC分析。
结论
这些发现为糖尿病肾病的早期治疗提供了理论依据。
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