Guirguis R, Schiffmann E, Liu B, Birkbeck D, Engel J, Liotta L
Laboratory of Pathology, National Cancer Institute, Bethesda, MD 20892.
J Natl Cancer Inst. 1988 Oct 5;80(15):1203-11. doi: 10.1093/jnci/80.15.1203.
Cell locomotion is an essential requirement for invasion and metastasis of malignant cells. We have previously described the characterization of a 50-kilodalton autocrine motility factor (AMF), a cytokine that stimulates motility in human tumor cells. In this study, we investigated the elaboration of this factor in vivo by human bladder carcinoma and in vitro by a cultured transitional cell carcinoma (TCC) of the bladder cell line T24P. Urine samples from patients with bladder cancer were assayed for their capacity to stimulate migration of tumor cells. Comparing all TCC cases (22 patients) with all nonmalignant diagnoses (27 patients), we found a statistically significant (P less than .001) difference in the motility values. Invasive TCC cases (15 patients) were significantly (P less than .002) higher in regard to motility values compared with noninvasive TCC cases (8 patients), including one case of carcinoma in situ. In follow-up screening studies evaluating TCC recurrence, the recurrent tumors (9 patients) were higher (P less than .001) in regard to motility values than the tumor-free cases (11 patients). Furthermore, T24P cells showed a dose-dependent motile response to their own serum-free conditioned medium as well as to the AMF present in the urine of TCC patients. This finding is consistent with the source of AMF in the urine of these patients being the cancer itself. An enzyme-linked immunosorbent assay (ELISA) for AMF was also developed. Values determined by ELISA correlated well with the motility values measured separately. These data support the potential usefulness of AMF as a urine marker for bladder TCC.
细胞运动是恶性细胞侵袭和转移的必要条件。我们之前已经描述了一种50千道尔顿自分泌运动因子（AMF）的特性，它是一种刺激人类肿瘤细胞运动的细胞因子。在本研究中，我们调查了这种因子在体内由人膀胱癌产生的情况，以及在体外由膀胱细胞系T24P的培养移行细胞癌（TCC）产生的情况。对膀胱癌患者的尿液样本进行检测，以评估其刺激肿瘤细胞迁移的能力。将所有TCC病例（22例患者）与所有非恶性诊断病例（27例患者）进行比较，我们发现运动值存在统计学上的显著差异（P小于0.001）。侵袭性TCC病例（15例患者）的运动值显著高于非侵袭性TCC病例（8例患者），包括1例原位癌，差异有统计学意义（P小于0.002）。在评估TCC复发的随访筛查研究中，复发肿瘤（9例患者）的运动值高于无肿瘤病例（11例患者），差异有统计学意义（P小于0.001）。此外，T24P细胞对其自身的无血清条件培养基以及TCC患者尿液中存在的AMF表现出剂量依赖性运动反应。这一发现与这些患者尿液中AMF的来源是癌症本身相一致。还开发了一种针对AMF的酶联免疫吸附测定（ELISA）。ELISA测定的值与单独测量的运动值相关性良好。这些数据支持AMF作为膀胱TCC尿液标志物的潜在实用性。
