Hirai K, Shimada H, Ogawa T, Taji S
Department of Anatomy, Kanazawa Medical University, Ishikawa, Japan.
Clin Exp Metastasis. 1991 Nov-Dec;9(6):517-27. doi: 10.1007/BF01768580.
The cell spreading ability of human lung cancer cells on collagen substrata was examined in comparison with normal human tracheal epithelial cells. Plastic dishes or multiwells were coated with type I, III or IV collagen gel at a concentrate of 200 micrograms/cm2. Ninety per cent of the normal cells were round on all collagens. Adenocarcinoma RERF-LC-MS and VMRC-LCD cell lines and squamous cell carcinoma VMRC-LCP cell line, which metastasize weakly after intrasplenic transplantation in nude mice, spread relatively poorly. Adenocarcinoma, A549 and SK-LU-1 and squamous cell carcinoma Calu-1 cell lines, which were highly metastatic to liver, spread well. Adenocarcinoma ABC-1 cell line, which is moderately metastatic to liver in nude mice, spread moderately. On type III collagen, three adenocarcinoma cell lines (A549, ABC-1 and VMRC-LCD) gradually started to contract after initial spreading and became round at 24 h. These results suggest that there may be a correlation between the degree of malignancy of human lung cancer cells and their spreading ability on collagen substrata, and that the cell spreading ability may be regulated by type III collagen in some lung cancer cells.
将人肺癌细胞与正常人气管上皮细胞相比，检测其在胶原蛋白基质上的细胞铺展能力。用浓度为200微克/平方厘米的I型、III型或IV型胶原凝胶包被塑料培养皿或多孔板。在所有胶原蛋白上，90%的正常细胞呈圆形。脾内移植到裸鼠后转移较弱的腺癌RERF-LC-MS和VMRC-LCD细胞系以及鳞状细胞癌VMRC-LCP细胞系，其铺展相对较差。向肝脏高度转移的腺癌A549和SK-LU-1以及鳞状细胞癌Calu-1细胞系，铺展良好。在裸鼠中向肝脏中度转移的腺癌ABC-1细胞系，铺展程度中等。在III型胶原蛋白上，三种腺癌细胞系（A549、ABC-1和VMRC-LCD）在初始铺展后逐渐开始收缩，并在24小时时变圆。这些结果表明，人肺癌细胞的恶性程度与其在胶原蛋白基质上的铺展能力之间可能存在相关性，并且在某些肺癌细胞中，细胞铺展能力可能受III型胶原蛋白调节。
