Nabeta Keisuke, Watanabe Satoru, Chibazakura Taku, Zendo Takeshi, Sonomoto Kenji, Shimizu-Kadota Mariko, Yoshikawa Hirofumi
Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Laboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Biosci Microbiota Food Health. 2019;38(3):111-114. doi: 10.12938/bmfh.18-030. Epub 2019 May 8.
Phosphoketolase (PK) is responsible for heterolactic fermentation; however, the PK gene of QU 25, , is transcribed constitutively, even under homolactic fermentation conditions. In order to deduce the regulatory mechanisms of PK activity in QU 25, XfpA levels in QU 25 cells under hetero- and homolactic fermentation conditions were tested using western blotting. The results showed that the XfpA protein expression was similar under both conditions and that the expression products formed complexes, most likely homodimers, indicating that the regulation of PK activity is downstream of translation.
磷酸酮醇酶（PK）负责异型乳酸发酵；然而，QU 25的PK基因即使在同型乳酸发酵条件下也持续转录。为了推断QU 25中PK活性的调控机制，利用蛋白质免疫印迹法检测了异型和同型乳酸发酵条件下QU 25细胞中XfpA的水平。结果表明，两种条件下XfpA蛋白表达相似，且表达产物形成复合物，很可能是同型二聚体，这表明PK活性的调控发生在翻译下游。
