Watanabe Satoru, Sato Masumi, Nimura-Matsune Kaori, Chibazakura Taku, Yoshikawa Hirofumi
Department of Bioscience, Tokyo University of Agriculture, Japan.
Biosci Biotechnol Biochem. 2007 Jan;71(1):279-82. doi: 10.1271/bbb.60647. Epub 2007 Jan 7.
Three dnaK and four dnaJ genes have been identified in the genome of cyanobacterium Synechococcus elongatus PCC 7942. Our comprehensive analysis of yeast two-hybrid screening revealed a specific interaction among DnaK2, DnaJ2, and RNase E, an essential endoribonuclease. We examined the effects of DnaK2 and DnaJ2 on RNase E activity by monitoring the digestion of psbAII transcript in vitro. The addition of DnaK2 and DnaJ2 obviously inhibited RNase E activity in an ATP-dependent manner. These results suggest that DnaK2 and DnaJ2 are involved in RNA degradation through interaction with RNase E.
在聚球藻属蓝细菌细长聚球藻PCC 7942的基因组中已鉴定出三个dnaK基因和四个dnaJ基因。我们对酵母双杂交筛选的全面分析揭示了DnaK2、DnaJ2和核糖核酸酶E（一种必需的内切核糖核酸酶）之间的特异性相互作用。我们通过监测体外psbAII转录本的消化来研究DnaK2和DnaJ2对核糖核酸酶E活性的影响。添加DnaK2和DnaJ2明显以ATP依赖的方式抑制核糖核酸酶E的活性。这些结果表明，DnaK2和DnaJ2通过与核糖核酸酶E相互作用参与RNA降解。
