Liao Yiyi, Hou Yuge, Zhong Yan, Chen Hong, Xu Chang, Tsunoda Makoto, Zhang Yingxia, Deng Shiming, Song Yanting
1Key Laboratory of Tropical Biological Resources of Ministry of Education; Department of Pharmaceutical Sciences, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, 570228 China.
2Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, 113-0033 Japan.
BMC Chem. 2019 May 10;13(1):61. doi: 10.1186/s13065-019-0578-7. eCollection 2019 Dec.
In this study, a simple one-step ionic liquid-based ultrasound-assisted dispersive liquid-liquid microextraction technique was coupled with high-performance liquid chromatography for the analysis of four pyrethroids in three kinds of traditional Chinese medicine oral liquid preparations: simotang oral liquid, kangbingdu oral liquid, and huaji oral liquid. The extraction parameters were examined to improve extraction efficiency. The optimum extraction conditions were 50 μL of 1-octyl-3-methylimidazolium hexafluorophosphate utilized as the extraction solvent and 800 μL of acetonitrile applied as the dispersive solvent. The extraction was assisted by ultrasonication for 8 min. The limits of detection for the four pyrethroids were within 0.007-0.024 mg L, and the limits of quantitation ranged between 0.023 and 0.080 mg L. The accuracy of the pyrethroid determination ranged from 80.1 to 106.4%. It was indicated that the proposed ionic liquid-based ultrasound-assisted dispersive liquid-liquid microextraction method had an easy operation and was accurate and environmentally friendly. This approach has potential for the analysis of pyrethroids in traditional Chinese medicine oral liquid preparations.
在本研究中，一种基于离子液体的简单一步超声辅助分散液液微萃取技术与高效液相色谱联用，用于分析三种中药口服液制剂（四磨汤口服液、抗病毒口服液和化积口服液）中的四种拟除虫菊酯。对萃取参数进行了考察以提高萃取效率。最佳萃取条件为：使用50 μL 1-辛基-3-甲基咪唑六氟磷酸盐作为萃取溶剂，800 μL乙腈作为分散溶剂。萃取过程通过超声处理8分钟辅助进行。四种拟除虫菊酯的检测限在0.007 - 0.024 mg/L范围内，定量限在0.023至0.080 mg/L之间。拟除虫菊酯测定的准确度在80.1%至106.4%之间。结果表明，所提出的基于离子液体的超声辅助分散液液微萃取方法操作简便、准确且环保。该方法在中药口服液制剂中拟除虫菊酯的分析方面具有潜力。
