通过钼硫化物量子点对叶酸荧光的猝灭作用测定叶酸。
Determination of folic acid via its quenching effect on the fluorescence of MoS quantum dots.
机构信息
State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, National Demonstration Center for Experimental Chemistry Education, College of Chemistry and Chemical Engineering, Ningxia University, Yinchuan, 750021, Ningxia, Hui Autonomous Region, People's Republic of China.
出版信息
Mikrochim Acta. 2019 Aug 5;186(9):605. doi: 10.1007/s00604-019-3705-1.
Molybdenum disulfide quantum dots (MoS QDs) are used in a fluorometric method for the determination of folic acid (FA) based on fluorescence quenching. The MoS QDs synthesized by a hydrothermal method possess bright blue fluorescence (with excitation/emission maxima of 325/415 nm), quantum yield of 3.7%, and excellent storage stability in solution (30 days in the refrigerator). Their fluorescence is quenched by FA, and intensity decreases linearly in the 0.1 to 125 μM FA concentration range. The detection limit is 0.1 μM (at S/N = 3), and the relative standard deviation (for n = 5) is 2.8% for 25 μM concentrations of FA. Studies on the quenching mechanism suggest that the effect is due to static quenching. The FA in commercial FA tablets was successfully determined. Graphical abstract Schematic representation of the hydrothermal method for the preparation of molybdenum disulfide quantum dots (MoS QDs) with about 2.7 ± 0.5 nm diameter using NaMoO and L-cysteine as Mo and S sources, and the fluorescence method for the determination of folic acid (FA) based on fluorescence quenching of MoS QDs.
二硫化钼量子点（MoS QDs）用于基于荧光猝灭的荧光法测定叶酸（FA）。水热法合成的 MoS QDs 具有明亮的蓝色荧光（激发/发射最大值为 325/415nm），量子产率为 3.7%，在溶液中具有优异的储存稳定性（在冰箱中 30 天）。它们的荧光被 FA 猝灭，在 0.1 到 125μM FA 浓度范围内强度线性降低。检测限为 0.1μM（S/N=3），对于 25μM FA 浓度，相对标准偏差（n=5）为 2.8%。对猝灭机制的研究表明，这种效应是由于静态猝灭引起的。成功测定了市售 FA 片剂中的 FA。示意图 用 NaMoO 和 L-半胱氨酸作为 Mo 和 S 源，通过水热法制备直径约为 2.7±0.5nm 的二硫化钼量子点（MoS QDs）的示意图，以及基于 MoS QDs 荧光猝灭的荧光法测定叶酸（FA）。
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