Department of Physics, Department of Materials Science and Engineering, and Department of Biomedical Engineering , City University of Hong Kong , Tat Chee Avenue , Kowloon , Hong Kong.
Division of Biomedical Engineering, Department of Chemical and Biological Engineering , The Hong Kong University of Science and Technology , Sai Kung , Hong Kong.
ACS Appl Mater Interfaces. 2019 Sep 4;11(35):31605-31614. doi: 10.1021/acsami.9b09066. Epub 2019 Aug 20.
Many postsurgical complications stem from bacteria colony formation on the surface of implants, but the usage of antibiotic agents may cause antimicrobial resistance. Therefore, there is a strong demand for biocompatible materials with an intrinsic antibacterial resistance not requiring extraneous chemical agents. In this study, homogeneous nanocones were fabricated by oxygen plasma etching on the surface of natural, biocompatible Bombyx mori silk films. The new hydroxyl bonds formed on the surface of the nanopatterned film by plasma etching increased the surface energy by around 176%. This hydrophilic nanostructure reduced the bacterial attachment by more than 90% for both Gram-negative () and Gram-positive () bacteria and at the same time improved the proliferation of osteoblast cells by 30%. The nanoengineered substrate and pristine silk were cultured for 6 h with three different bacteria concentrations of 10, 10, and 10 CFU mL and the cell proliferation on the nanopatterned samples was significantly higher due to limited bacteria attachment and prevention of biofilm formation. The concept and materials described here reveal a promising alternative to produce biomaterials with an inherent biocompatibility and bacterial resistance simultaneously to mitigate postsurgical infections and minimize the use of antibiotics.
许多手术后并发症源于植入物表面细菌菌落的形成，但抗生素的使用可能会导致抗微生物耐药性。因此，人们强烈需要具有内在抗菌性的生物相容性材料，而不需要外来的化学试剂。在这项研究中，通过在天然生物相容性的桑蚕丝膜表面进行氧等离子体蚀刻，制备出均匀的纳米锥形结构。等离子体蚀刻在纳米图案化薄膜表面形成的新的羟基键使表面能增加了约 176%。这种亲水纳米结构使革兰氏阴性菌（）和革兰氏阳性菌（）的细菌附着减少了 90%以上，同时使成骨细胞的增殖提高了 30%。纳米工程化的基底和原始丝在三种不同的细菌浓度（10、10 和 10 CFU mL）下培养了 6 小时，由于细菌附着有限和防止生物膜形成，因此纳米图案化样品上的细胞增殖明显更高。这里描述的概念和材料揭示了一种很有前途的方法，可以同时生产具有内在生物相容性和抗菌性的生物材料，以减轻手术后感染并尽量减少抗生素的使用。
