从人外周血中分离出的B和T淋巴细胞的5'-核苷酸酶
5'-nucleotidase of B and T lymphocytes isolated from human peripheral blood.
作者信息
Rowe M, de Gast C G, Platts-Mills T A, Asherson G L, Webster A D, Johnson S M
出版信息
Clin Exp Immunol. 1979 Apr;36(1):97-101.
Abstract
The ecto-5'-nucleotidase activities of highly purified T and B lymphocytes from human peripheral blood have been investigated using biochemical and histochemical techniques. The enzyme activity of the purified B cells was about 3.5 times that of the T cells. Using a histochemical assay, 21--55% of the B cells stained positively for 5'-nucleotidase, but only 2--22% of the T cells were positive. These results are discussed in relation to the low 5'-nucleotidase activities found on peripheral blood lymphocytes from patients with chronic lymphatic leukaemia and some patients with primary hypogammaglobulinaemia.
摘要
利用生化和组织化学技术，对来自人外周血的高度纯化的T淋巴细胞和B淋巴细胞的外切5'-核苷酸酶活性进行了研究。纯化的B细胞的酶活性约为T细胞的3.5倍。采用组织化学分析方法，21%至55%的B细胞对5'-核苷酸酶呈阳性染色，但只有2%至22%的T细胞呈阳性。结合慢性淋巴细胞白血病患者和一些原发性低丙种球蛋白血症患者外周血淋巴细胞中发现的低5'-核苷酸酶活性，对这些结果进行了讨论。
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