Chen Yanyan, Zeng Benjuan, Shi Peng, Xiao Heng, Chen Shanyuan
Kunming Institute of Zoology, University of Chinese Academy of Sciences, Kunming 650203, China.
Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650204, China.
Animals (Basel). 2019 Aug 5;9(8):527. doi: 10.3390/ani9080527.
Previous studies have shown that Yunnan humped cattle have higher disease resistance than pure taurine cattle, such as Holsteins. However, there exists limited information about the molecular genetic basis underlying disease resistance differences between them. The objective of this study was to compare differentially expressed genes (DEGs) in the liver and spleen tissues of Holstein and Yunnan humped cattle through comparative transcriptome analysis, using RNA-sequencing. In total, 1564 (647 up- and 917 down-regulated genes) and 1530 (716 up- and 814 down-regulated genes) DEGs were obtained in the liver and spleen tissues of Holstein and Yunnan humped cattle comparison groups, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the DEGs were mainly associated with the RIG-I signaling pathway, immune responses, major histocompatibility complex (MHC) class I protein complex and complement activation, human T-cell lymphotropic virus type-I (HTLV-I) infection. Some genes related to immune function, such as , , , , , , and , were up-regulated in Yunnan humped cattle, while , , , , , and were down-regulated. The expression levels of 11 selected DEGs, analyzed by quantitative reverse-transcription polymerase chain reaction (RT-qPCR), were consistent with the deep sequencing results by RNA-sequencing. Our results will provide a scientific basis and key technical support for disease-resistant breeding of domestic cattle.
先前的研究表明，云南瘤牛比纯种普通牛（如荷斯坦牛）具有更高的抗病性。然而，关于它们之间抗病性差异的分子遗传基础的信息有限。本研究的目的是通过RNA测序的比较转录组分析，比较荷斯坦牛和云南瘤牛肝脏和脾脏组织中的差异表达基因（DEG）。在荷斯坦牛和云南瘤牛比较组的肝脏和脾脏组织中，分别获得了1564个（647个上调基因和917个下调基因）和1530个（716个上调基因和814个下调基因）DEG。基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析表明，DEG主要与RIG-I信号通路、免疫反应、主要组织相容性复合体（MHC）I类蛋白复合体和补体激活、人类I型嗜T细胞病毒（HTLV-I）感染有关。一些与免疫功能相关的基因，如 、 、 、 、 、 和 ，在云南瘤牛中上调，而 、 、 、 、 和 下调。通过定量逆转录聚合酶链反应（RT-qPCR）分析的11个选定DEG的表达水平与RNA测序的深度测序结果一致。我们的结果将为家牛抗病育种提供科学依据和关键技术支持。
