沉默环状 RNA circZNF609 通过上调前列腺癌细胞中的 microRNA-186-5p 抑制其生长、迁移和侵袭。
Silencing circular RNA circZNF609 restrains growth, migration and invasion by up-regulating microRNA-186-5p in prostate cancer.
机构信息
a Department of Urology, The First Affiliated Hospital of Harbin Medical University , Harbin , China.
b Department of Reproductive Medicine, The First Affiliated Hospital of Harbin Medical University , Harbin , China.
出版信息
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3350-3358. doi: 10.1080/21691401.2019.1648281.
Prostate cancer (PC) fearfully impacts men's health. We explored the efficacy and mechanism of circular RNA circZNF609 (circZNF609) on colony formation, viability, apoptosis, migration and invasion and in PC cells. Colony formation, CCK-8, flow cytometry, migration and invasion assay were respectively used to detect the functions of circZNF609 and microRNA (miR)-186-5p on cell colony ability, viability, apoptosis, migration and invasion. circZNF609 and miR-186-5p expression were changed by cell transfection and tested by RT-qPCR. Moreover, Cleaved-Caspase-3, Cleaved-Caspase-9, matrix metalloproteinase-9 (MMP-9), Vimentin and relate-proteins of cell pathways were examined through Western blot. circZNF609 was highly expressed at PC tissues. circZNF609 declined cell colony ability, viability, migration and invasion and caused apoptosis. Furthermore, circZNF609 negatively regulated miR-186-5p, miR-186-5p inhibitor could reverse impacts of circZNF609. Finally, circZNF609 restrained the YAP1 and AMPK pathways by up-regulating miR-186-5p. Silencing circZNF609 restrained growth, migration and invasion of PC cells by up-regulating miR-186-5p via YAP1 and AMPK pathways. Highlights circZNF609 is highly expressed in PC tissues; circZNF609 restrains cell growth, migration and invasion; circZNF609 exerts its function by up-regulating miR-186-5p; circZNF609 exerts its function by YAP1 and AMPK signaling pathways.
前列腺癌（PC）严重影响男性健康。我们探索了环状 RNA circZNF609（circZNF609）对PC 细胞集落形成、活力、凋亡、迁移和侵袭的功效和机制。分别使用集落形成、CCK-8、流式细胞术、迁移和侵袭测定来检测 circZNF609 和 microRNA（miR）-186-5p 对细胞集落能力、活力、凋亡、迁移和侵袭的作用。通过细胞转染改变 circZNF609 和 miR-186-5p 的表达，并通过 RT-qPCR 进行测试。此外，通过 Western blot 检测细胞通路中的Cleaved-Caspase-3、Cleaved-Caspase-9、基质金属蛋白酶-9（MMP-9）、波形蛋白和相关蛋白。circZNF609 在 PC 组织中高表达。circZNF609 降低了细胞集落能力、活力、迁移和侵袭，并导致凋亡。此外，circZNF609 负调控 miR-186-5p，miR-186-5p 抑制剂可以逆转 circZNF609 的影响。最后，circZNF609 通过上调 miR-186-5p 来抑制 YAP1 和 AMPK 通路。沉默 circZNF609 通过上调 miR-186-5p 来抑制 PC 细胞的生长、迁移和侵袭，通过 YAP1 和 AMPK 信号通路。亮点 circZNF609 在 PC 组织中高表达；circZNF609 抑制细胞生长、迁移和侵袭；circZNF609 通过上调 miR-186-5p 发挥作用；circZNF609 通过 YAP1 和 AMPK 信号通路发挥作用。
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