Department of Chemistry, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, 200433, China.
Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
Anal Chim Acta. 2019 Nov 4;1079:111-119. doi: 10.1016/j.aca.2019.06.045. Epub 2019 Jun 24.
Developing an effective strategy for endogenous phosphopeptides enrichment and separation is necessary in phosphopeptidomics analysis due to the serious inference caused by chaotic biological environment. In this work, a size-exclusive magnetic binary metal mesoporous nanocomposites were synthesized to capture phosphorylated peptides for mass spectrometry analysis. The novel FeO@TiO-ZrO@mSiO nanocomposites possessed the merits of ordered mesoporous channels, superparamagnetism and the integration of dual affinity of Zr-O and Ti-O. Compared with single-metal centered nanocomposites (FeO@TiO@mSiO and FeO@ZrO@mSiO), FeO@TiO-ZrO@mSiO showing much enhanced enrichment performance towards mono- and multi-phospho-peptides with better sensitivity. With all the advances, the FeO@TiO-ZrO@mSiO was also successfully applied to capture endogenous phosphorylated peptides from human saliva. And consequently, a total of 30 phosphopeptides containing 2 multi- and 28 mono-phosphopeptides were identified efficiently. These results show that the novel materials have the great potential in peptidome analysis.
由于混沌的生物环境会造成严重干扰，在磷酸肽组学分析中，开发一种有效的内源性磷酸肽富集和分离策略是必要的。在这项工作中，我们合成了一种尺寸排阻的磁性双金属介孔纳米复合材料，用于捕获磷酸化肽进行质谱分析。新型 FeO@TiO-ZrO@mSiO 纳米复合材料具有有序介孔通道、超顺磁性和 Zr-O 与 Ti-O 双重亲和性的优点。与单金属中心纳米复合材料（FeO@TiO@mSiO 和 FeO@ZrO@mSiO）相比，FeO@TiO-ZrO@mSiO 对单磷酸肽和多磷酸肽具有更好的灵敏度和增强的富集性能。由于所有这些进展，FeO@TiO-ZrO@mSiO 还成功地用于从人唾液中捕获内源性磷酸化肽。结果，有效地鉴定出了 30 种磷酸肽，其中含有 2 种多磷酸肽和 28 种单磷酸肽。这些结果表明，这种新型材料在肽组学分析中有很大的应用潜力。
