Department of Chemistry and The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, 200438, China.
School of Pharmacy, Fudan University, Shanghai, 200438, China.
Talanta. 2020 Jan 15;207:120313. doi: 10.1016/j.talanta.2019.120313. Epub 2019 Sep 6.
Peptidomics research is of great significance for discovering potential biomarkers and monitoring human diseases. As a kind of common clinical biofluid, saliva known for its noninvasive collection and easy accessibility has been widely used in peptidomics research. In this article, we combined immobilized metal ions affinity chromotography (IMAC) with mesoporous material and proposed the copper ion doped magnetic mesoporous silica material (denoted as FeO@mSiO-Cu) which had a large surface area of 221 m g and pore volume of 0.20 cm g. By immobilizing copper ions onto the mesopore walls, the standard peptide Angiotensin II could be identified in an extremely low concentration of 0.1 fmol μl and in a mass ratio of 1:500 (Angiotensin II:BSA, m/m), which indicated significant sensitivity and a great size-exclusive ability. In addition, the introduction of polydopamine (PDA) made FeO@mSiO-Cu more hydrophilic and biocompatible which could improve the profiling of endogenous peptides in bio-sample. Finally, 131 endogenous peptides were identified in human saliva after enrichment with FeO@mSiO-Cu. Therefore, FeO@mSiO-Cu nanoparticles provided a promising candidate protocol for biomarker discovery.
肽组学研究对于发现潜在的生物标志物和监测人类疾病具有重要意义。唾液作为一种常见的临床生物体液，以其非侵入性采集和易于获取的特点，已被广泛应用于肽组学研究。在本文中，我们结合固载金属离子亲和层析（IMAC）和介孔材料，提出了铜离子掺杂磁性介孔硅材料（FeO@mSiO-Cu），其比表面积高达 221m²/mg，孔体积为 0.20cm³/g。通过将铜离子固定在介孔壁上，该材料能够在极其低的浓度（0.1 fmol/μl）和质量比（1:500（血管紧张素 II:BSA，m/m））下识别标准肽血管紧张素 II，表现出显著的灵敏度和出色的尺寸排他性。此外，聚多巴胺（PDA）的引入使 FeO@mSiO-Cu 具有更好的亲水性和生物相容性，从而提高了生物样品中内源性肽的分析能力。最后，经 FeO@mSiO-Cu 富集后，从人唾液中鉴定出 131 种内源性肽。因此，FeO@mSiO-Cu 纳米粒子为生物标志物的发现提供了一种有前途的候选方案。
