Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
Anal Chim Acta. 2019 Nov 4;1079:164-170. doi: 10.1016/j.aca.2019.06.042. Epub 2019 Jun 22.
Ultrathin two-dimensional (2D) metal-organic framework (MOF) nanosheets have attracted numerous attention due to their unparalleled advantages such as unique structures, large surface areas, good stability and high catalytic efficiency. In this paper, 2D Cu(bpy)(OTf) nanosheets (Cu-MOF, bpy = 4,4-bipyridine, OTf = trifluoromethanesulfonate) are first utilized to achieve the fluorescent detection for HO and glucose with their intrinsic peroxidase-like activity. The fluorescent biosensor Cu-MOF nanosheets were achieved by the efficient catalysis of non-fluorescent thiamine (TH) to strong fluorescent thiochrome in the presence of HO. With the combination of glucose oxidase, highly selective and sensitive fluorescent detection for glucose could be established with a detection limit of 0.41 μM and two separate linear ranges of 10-100 μM and 100-1000 μM, respectively. Furthermore, the developed method has been applied to human serum for the quantitative determination of glucose as a clinical diagnosis indicator of diabetes mellitus.
超薄二维（2D）金属-有机骨架（MOF）纳米片由于其独特的结构、大的表面积、良好的稳定性和高催化效率等无与伦比的优势而引起了广泛关注。在本文中，首次利用 2D Cu(bpy)(OTf)纳米片（Cu-MOF，bpy=4,4-联吡啶，OTf=三氟甲磺酸根）利用其固有的过氧化物酶样活性实现对 HO 和葡萄糖的荧光检测。在 HO 的存在下，荧光生物传感器 Cu-MOF 纳米片通过高效催化无荧光硫胺素（TH）生成强荧光噻嗪。结合葡萄糖氧化酶，可以建立对葡萄糖的高选择性和灵敏的荧光检测，检测限为 0.41 μM，两个独立的线性范围分别为 10-100 μM 和 100-1000 μM。此外，该方法已应用于人血清中葡萄糖的定量测定，作为糖尿病的临床诊断指标。
