Wang Junning, Wei Tianxiang, Liu Yichen, Bao Mengyuan, Feng Rui, Qian Yuxuan, Yang Xue, Si Ling, Dai Zhihui
Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, People's Republic of China.
Analyst. 2020 Apr 21;145(8):3002-3008. doi: 10.1039/c9an02418b. Epub 2020 Mar 4.
Metal-organic frameworks (MOFs) have attracted great attention as enzyme mimic materials in colorimetric hydrogen peroxide (HO) detection. At present, it is highly desirable but remains challenging to prepare MOFs with high stability and dispersity to further improve their peroxidase-mimicking catalytic activity. In this work, we developed a new and facile method for the synthesis of a sub-100 nm peroxidase-mimicking zirconium porphyrin metal-organic framework (Zr-PorMOF) via a solvothermal method. The experimental results indicated that compared with the micron-sized crystals obtained using a classical synthesis method, the catalytic activity, stability and dispersity in water of the colloidal Zr-PorMOF were obviously enhanced. The as-synthesized colloidal Zr-PorMOF was further successfully applied in colorimetric HO detection, and satisfactory detection performance was obtained. Furthermore, the colloidal Zr-PorMOF was also successfully employed in the construction of a peroxidase-based tandem catalysis system. Taking glucose oxidase as an example, this system was successfully applied for glucose sensing in real human serum samples, which proved its practical feasibility in diabetes diagnosis and indicates its high potential feasibility in peroxidase-related applications in complex biomatrix.
金属有机框架材料（MOFs）作为比色法检测过氧化氢（H₂O₂）的模拟酶材料受到了广泛关注。目前，制备具有高稳定性和分散性的MOFs以进一步提高其模拟过氧化物酶的催化活性是非常有必要的，但仍具有挑战性。在这项工作中，我们开发了一种新的简便方法，通过溶剂热法合成了一种亚100纳米的模拟过氧化物酶的锆卟啉金属有机框架材料（Zr-PorMOF）。实验结果表明，与使用经典合成方法获得的微米级晶体相比，胶体Zr-PorMOF在水中的催化活性、稳定性和分散性明显增强。所合成的胶体Zr-PorMOF进一步成功应用于比色法检测H₂O₂，并获得了令人满意的检测性能。此外，胶体Zr-PorMOF还成功用于构建基于过氧化物酶的串联催化系统。以葡萄糖氧化酶为例，该系统成功应用于实际人血清样品中的葡萄糖传感，证明了其在糖尿病诊断中的实际可行性，并表明其在复杂生物基质中过氧化物酶相关应用中的高潜在可行性。
