Institute of Molecular Medicine, State Key Laboratory of Oncogenes and Related Genes, Shanghai Institute of Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 200011, Shanghai, China.
Department of Ophthalmology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200011, Shanghai, China.
Nat Commun. 2019 Aug 6;10(1):3452. doi: 10.1038/s41467-019-11390-8.
Bacteria have been extensively utilized for bioimaging, diagnosis and therapy given their unique characteristics including genetic manipulation, rapid proliferation and disease site targeting specificity. However, clinical translation of bacteria for these applications has been largely restricted by their unavoidable side effects and low treatment efficacies. Engineered bacteria for biomedical applications ideally need to generate only a low inflammatory response, show slow elimination by macrophages, low accumulation in normal organs, and almost unchanged inherent bioactivities. Here we describe a set of stealth bacteria, cell membrane coated bacteria (CMCB), meeting these requirement. Our findings are supported by evaluation in multiple mice models and ultimately demonstrate the potential of CMCB to serve as efficient tumor imaging agents. Stealth bacteria wrapped up with cell membranes have the potential for a myriad of bacterial-mediated biomedical applications.
鉴于其独特的特性，包括遗传操作、快速增殖和疾病部位靶向特异性，细菌已被广泛用于生物成像、诊断和治疗。然而，细菌在这些应用中的临床转化在很大程度上受到其不可避免的副作用和低治疗效果的限制。用于生物医学应用的工程细菌理想情况下只需产生低炎症反应，被巨噬细胞缓慢清除，在正常器官中低积累，并几乎保持不变的固有生物活性。在这里，我们描述了一组满足这些要求的隐形细菌，细胞膜包被的细菌（CMCB）。我们的研究结果得到了多种小鼠模型评估的支持，并最终证明了 CMCB 作为有效肿瘤成像剂的潜力。用细胞膜包裹的隐形细菌有可能应用于各种细菌介导的生物医学应用。
