Institute of Molecular Medicine, State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Department of Ophthalmology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China.
Nat Commun. 2019 Dec 19;10(1):5783. doi: 10.1038/s41467-019-13727-9.
The gut microbiota represents a huge community of microorganisms that play essential roles in immune modulation and homeostasis maintenance. Microbiota transplantation is an important approach to prevent and treat disease as it can inhibit pathogen colonization and positively modulate bacterial composition. However, the development of oral bacterial therapeutics has been restricted by low bioavailability and limited retention in the gastrointestinal tract. Here, we report a simple yet highly efficient method to coat gut microbes via biointerfacial supramolecular self-assembly. Coating can be performed within 15 min by simply vortexing with biocompatible lipids. Bacteria coated with an extra self-assembled lipid membrane exhibit significantly improved survival against environmental assaults and almost unchanged viability and bioactivity. We demonstrate their enhanced efficacies in oral delivery and treatment using two murine models of colitis. We suggest that biointerfacial supramolecular self-assembly may provide a unique platform to generate advanced bacterial therapeutics for the treatment of various diseases.
肠道微生物群代表了一个庞大的微生物群落，它们在免疫调节和维持体内平衡方面发挥着重要作用。菌群移植是预防和治疗疾病的一种重要方法，因为它可以抑制病原体定植并积极调节细菌组成。然而，口服细菌治疗药物的发展受到生物利用度低和在胃肠道中保留时间有限的限制。在这里，我们报告了一种通过生物界面超分子自组装来包被肠道微生物的简单而高效的方法。通过与生物相容性脂质简单涡旋，在 15 分钟内即可完成包被。用额外的自组装脂质膜包被的细菌对环境攻击的生存能力显著提高，而活力和生物活性几乎不变。我们使用两种结肠炎的小鼠模型证明了它们在口服递送和治疗中的增强功效。我们认为生物界面超分子自组装可能为治疗各种疾病的先进细菌治疗药物提供独特的平台。
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