Kleiner U, Trenner P
Institut für angewandte Tierhygiene Eberswalde beim Ministerium für Land-, Forst- und Nahrungsgüterwirtschaft der DDR.
Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med. 1988 May;186(2):138-52.
The intention of investigations was to clear central problems of disinfecting testing in carrier model. Ways of practical and standardized evaluation should be found out. Concrete investigations were made to guarantee a highly and regularly initial bacterial count, practical germ carriers and organic matter as also detecting damaged bacteria. Investigations for resistance level of test organisms were made in suspension test with formaldehyde as reference disinfectant. It was obviously that test organisms used in disinfecting testing were after 8 weeks more sensitive against disinfectants. Therefore results of disinfecting testing are often no representative because those test organisms are not qualified for representative declaration of disinfectant efficiency. Animal passage in distance of 8 weeks is a possibility for guaranteeing highly and regularly resistance of microorganisms We recommend to use soft wood from the same origin, addition of 10% horse serum and 1% carboxymethylcellulose as organic matter for a model relating to practice. Buffered peptone water and nutrient agar are guarantee for detecting damaged bacteria after disinfecting.
研究的目的是解决载体模型中消毒测试的核心问题。应找出实际且标准化的评估方法。进行了具体研究，以确保初始细菌计数高且稳定、有实际的细菌载体和有机物，同时检测受损细菌。以甲醛作为参考消毒剂，通过悬液试验对试验微生物的抗性水平进行了研究。很明显，消毒测试中使用的试验微生物在8周后对消毒剂更敏感。因此，消毒测试结果往往没有代表性，因为这些试验微生物不符合消毒剂效果代表性声明的要求。每隔8周进行动物传代是保证微生物具有高且稳定抗性的一种方法。我们建议使用来自同一来源的软木，添加10%马血清和1%羧甲基纤维素作为与实际相关模型的有机物。缓冲蛋白胨水和营养琼脂可保证消毒后检测受损细菌。
