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[兽用消毒剂细菌学有效性检测模型]

[Model of bacteriologic effectiveness testing of disinfectants for veterinary medicine].

作者信息

Kleiner U, Trenner P

机构信息

Institut für angewandte Tierhygiene Eberswalde beim Ministerium für Land, Forst- und Nahrungs-güterwirtschaft der DDR.

出版信息

Arch Exp Veterinarmed. 1990;44(1):103-16.

PMID:2188627
Abstract

Proper evaluation of effectiveness of chemical disinfectants depends on availability of a standardised test methodology which can provide reproducible values. The binding rules and regulations for testing and acceptance under the heading of "Methods for Testing Chemical Disinfectants for Veterinary Medicine" (team of authors, 1987) have resulted from years of experimental research in the field of disinfectant development. They include a model for bacteriological laboratory testing which is described in this paper, with complementary reference being made to experimental planning and appraisal of disinfection success. An account is given of preparatory testing, using agar diffusion and serial dilution tests, and of main testing, using suspension and microorganism carrier tests. The RF% microorganism reduction rate is introduced for appraisal of disinfection success. With differentiated initial germ counts, the comparability of experimental results provided by RF% has proved to be superior to that obtainable from conventional calculation.

摘要

化学消毒剂有效性的正确评估取决于能否获得可提供可重复值的标准化测试方法。“兽用化学消毒剂测试方法”(作者团队,1987年)标题下的测试和验收的约束规则与条例源于消毒剂开发领域多年的实验研究。它们包括本文所述的细菌学实验室测试模型,并对消毒成功的实验规划和评估进行了补充参考。介绍了使用琼脂扩散和连续稀释试验的预备测试以及使用悬浮液和微生物载体试验的主要测试。引入了RF%微生物减少率来评估消毒成功与否。在初始菌数不同的情况下,已证明RF%提供的实验结果的可比性优于传统计算方法得出的结果。

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