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通过高效阴离子交换色谱、凝胶过滤色谱和疏水相互作用色谱法纯化炭疽毒素成分。

Purification of anthrax-toxin components by high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography.

作者信息

Quinn C P, Shone C C, Turnbull P C, Melling J

机构信息

Public Health Laboratory Service, Centre for Applied Microbiology and Research, Salisbury, Wilts, U.K.

出版信息

Biochem J. 1988 Jun 15;252(3):753-8. doi: 10.1042/bj2520753.

DOI:10.1042/bj2520753
PMID:3138975
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1149211/
Abstract

A procedure has been developed for purification of the tripartite anthrax-toxin components. This involves sequential high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography. From an initial culture volume of 15 litres, typical yields of 8 mg of protective antigen, 13 mg of lethal factor and 7 mg of oedema factor are produced to higher degrees of purity than have previously been achieved by conventional chromatographic techniques.

摘要

已开发出一种用于纯化炭疽三联体毒素成分的方法。该方法包括依次进行的高效阴离子交换色谱、凝胶过滤色谱和疏水相互作用色谱。从15升的初始培养体积中，可获得典型产量为8毫克的保护性抗原、13毫克的致死因子和7毫克的水肿因子，其纯度比以往通过传统色谱技术所达到的纯度更高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/8fed46c778bf/biochemj00229-0135-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/929ac34e2528/biochemj00229-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/867cb1d70459/biochemj00229-0132-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/4eda07a34620/biochemj00229-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/cfbd310cb079/biochemj00229-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/04d795672b12/biochemj00229-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/8fed46c778bf/biochemj00229-0135-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/929ac34e2528/biochemj00229-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/867cb1d70459/biochemj00229-0132-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/4eda07a34620/biochemj00229-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/cfbd310cb079/biochemj00229-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/04d795672b12/biochemj00229-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1b1/1149211/8fed46c778bf/biochemj00229-0135-b.jpg

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