在一种新的、特定的培养基中制备炭疽芽孢杆菌抗原。
Elaboration of Bacillus anthracis antigens in a new, defined culture medium.
作者信息
Ristroph J D, Ivins B E
出版信息
Infect Immun. 1983 Jan;39(1):483-6. doi: 10.1128/iai.39.1.483-486.1983.
Abstract
Improved culture conditions and a new, completely synthetic medium (R medium) were developed to facilitate the production of Bacillus anthracis holotoxin antigens. Levels of these antigens up to fivefold greater than the highest previously reported values were recovered with the described system. Cultures of Sterne, V770-NP1-R, and Vollum 1B strains of B. anthracis were monitored for growth, pH change, glucose utilization, supernatant protein concentration, lethal toxin activity, and protease activity.
摘要
为了促进炭疽芽孢杆菌全毒素抗原的生产，开发了改良的培养条件和一种全新的完全合成培养基（R培养基）。利用所描述的系统，这些抗原的产量比之前报道的最高值高出五倍。对炭疽芽孢杆菌的斯特恩、V770-NP1-R和沃勒姆1B菌株培养物进行了生长、pH变化、葡萄糖利用、上清液蛋白浓度、致死毒素活性和蛋白酶活性的监测。
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