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罗马尼亚 SANADOR 医院急性呼吸道感染患儿的鼻咽分子检测。

and nasopharyngeal molecular detection in children with acute respiratory tract infection in SANADOR Hospital, Romania.

机构信息

Faculty of Biology, University of Bucharest, Bucharest, Romania.

Molecular Biology Laboratory, SANADOR Hospital, Bucharest, Romania.

出版信息

J Med Microbiol. 2019 Oct;68(10):1466-1470. doi: 10.1099/jmm.0.001038. Epub 2019 Aug 7.

Abstract

. and are both commensals of the human nasopharynx with a high capacity to cause upper and lower respiratory tract infections. Molecular testing of nasopharyngeal samples from children at the primary care paediatric department presenting with acute respiratory tract infections (ARTIs). From June 2016 to May 2017, 156 nasopharyngeal swabs from children diagnosed with ARTIs who had been admitted to or followed up as outpatients at the Department of Paediatrics, SANADOR Hospital (Bucharest, Romania) were tested for the presence of , , , , , and DNA. had the highest detection rate (53.8 %, =84/156), followed by (41 %, =64/156) and co-detection (26.2 %, =41/156). A definitive laboratory diagnosis of these micro-organisms can be made for invasive disease, but there are difficulties in establishing the aetiology for mucosal infection. Molecular detection tests could complement culture-based tests by strengthening their surveillance.

摘要

. 和 都是人类鼻咽部的共生菌,具有引起上呼吸道和下呼吸道感染的高能力。对在初级保健儿科部门因急性呼吸道感染 (ARTI) 就诊或随诊的儿童的鼻咽样本进行分子检测。从 2016 年 6 月至 2017 年 5 月,对在罗马尼亚布加勒斯特 SANADOR 医院儿科部门住院或门诊随访的被诊断为 ARTI 的儿童的 156 个鼻咽拭子进行了 、 、 、 、 和 DNA 的检测。 的检出率最高(53.8%,=84/156),其次是 (41%,=64/156)和 共同检出(26.2%,=41/156)。这些微生物的明确实验室诊断可用于侵袭性疾病,但在确定粘膜感染的病因方面存在困难。分子检测试验可以通过加强监测来补充基于培养的检测。

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