在酵母中合成盘基网柄菌色氨酸转运核糖核酸（tRNATrp）的结构要求。

Structural requirements for the synthesis of tRNATrp from Dictyostelium discoideum in yeast.

作者信息

Dingermann T, Nerke K, Blöcker H, Frank R

机构信息

Institut für Biochemie der Medizinischen Fakultät, Universität Erlangen-Nürnberg, F.R.G.

出版信息

Biochimie. 1988 Jun;70(6):711-9. doi: 10.1016/0300-9084(88)90099-5.

DOI:10.1016/0300-9084(88)90099-5

PMID:3139087

Abstract

Dictyostelium tRNA genes can generally be expressed in vivo in yeast. Among tested Dictyostelium tRNA genes a tRNATrp gene containing a 13 bp intron is transcribed with particularly poor apparent efficiency and the intron is not removed. Elimination of the intron from the gene increases the amount of transcription products significantly. Splicing can only occur if minimal base-pairing of the anticodon with intron sequences is possible. Accumulation of tRNA gene transcripts decreases with the inability of intron splicing. Products of neither amber (UAG) nor opal (UGA) suppressor variants of the tRNATrp gene from Dictyostelium are able to suppress corresponding non-sense mutations in defined structural yeast genes. This also holds true for suppressor tRNA gene variants with precisely deleted intron regions.

摘要

盘基网柄菌的tRNA基因通常能够在酵母体内表达。在经过测试的盘基网柄菌tRNA基因中，一个含有13bp内含子的tRNATrp基因转录效率明显特别低，并且内含子未被去除。从该基因中去除内含子可显著增加转录产物的量。只有当反密码子与内含子序列能够进行最小程度的碱基配对时，剪接才会发生。随着内含子剪接能力的丧失，tRNA基因转录本的积累会减少。来自盘基网柄菌的tRNATrp基因的琥珀色（UAG）和乳白（UGA）抑制变体的产物均无法抑制特定结构酵母基因中的相应无义突变。对于内含子区域精确缺失的抑制性tRNA基因变体也是如此。