探讨迷迭香甲醇提取物对紫外线暴露皮肤衰老的潜在影响：体内和体外模型。

Exploring the Potential Effect of Methanolic Extract of Salvia officinalis Against UV Exposed Skin Aging: In vivo and In vitro Model.

机构信息

Department of Pharmacognosy, School of Pharmacy and Research, People's University, Bhopal (M.P.) 462037, India.

Pinnacle Biomedical Research Institute, Bhopal (M.P.) 462003, India.

出版信息

Curr Aging Sci. 2021;14(1):46-55. doi: 10.2174/1874609812666190808140549.

DOI:10.2174/1874609812666190808140549

PMID:31393258

Abstract

The medicinal plants have enormous pharmacological properties with fewer side effects. Today, there is an increasing demand of medicinal plants as an anti-aging and anti-wrinkle agent. ; Objective: The aim of this study is to evaluate the antioxidant, anti-aging and anti-wrinkle potential of Salvia officinalis. ; Materials and Methods: Salvia officinalis (Lamiaceae) is folk medicine of Asia and Latin America. Powdered crude drug 100 g was successively extracted in a soxhlet apparatus with petroleum ether (60-80ºC), chloroform and methanol. After successive solvents, extraction methanolic extract was used for testing of antioxidant potential using DPPH assay. Further, the antiaging potential of the extract was investigated by the inhibitory effect of various enzymatic estimations i.e. Col-I, Ela- I and Hya-I inhibitory assays on early aging human skin fibroblasts. The antiwrinkle potential of plant Salvia officinalis was done by using a UV light-induced photoaging model. ; Results: Phytochemical analysis showed the presence of glycosides, alkaloids flavonoids, and triterpenoids, saponins and Phenolic Compounds at high level. The extract showed inhibitory concentration (IC50: 24.65) and ascorbic acid. The standard antioxidant showed inhibitory concentration (IC50: 20.10). In enzymatic estimations assay, the Col-I, Ela-I and Hya-I of extract were assessed showing inhibitory concentration as Col-I (IC50:21.36), Ela-I (IC50:35.05) and Hya-I (IC50:23.44), respectively. Thus, MeOH extract of Salvia officinalis can inhibit 50% of the activity of aging-related enzymes Col-I, Ela-I and Hya-I. The wrinkle score of negative control i.e. UV treated group was 2.83 ± 0.408, and MeOH extract of Salvia officinalis treated group is 1.83 ± 0.753. ; Conclusion: This study concluded that MeOH extract of Salvia officinalis has confirmed the high antioxidant potential and in vitro and in vivo inhibitory potential of antiaging enzymes assessed, thus they could be used for further development of cosmetic products and nutraceuticals.

摘要

药用植物具有巨大的药理学特性，副作用较少。如今，人们对药用植物作为抗衰老和抗皱剂的需求日益增加。

目的

本研究旨在评估迷迭香的抗氧化、抗衰老和抗皱潜力。

材料和方法

迷迭香（唇形科）是亚洲和拉丁美洲的民间药物。将 100 克粗药粉在索氏提取器中用石油醚（60-80°C）、氯仿和甲醇依次提取。在使用不同溶剂提取后，用 DPPH 法测定甲醇提取物的抗氧化潜力。此外，还通过对早期衰老的人皮肤成纤维细胞的各种酶测定（即 Col-I、Ela-I 和 Hya-I 抑制测定）来研究提取物的抗衰老潜力。植物迷迭香的抗皱潜力是通过使用紫外线诱导的光老化模型来完成的。

结果

植物化学分析表明，该植物含有高水平的糖苷、生物碱、类黄酮、三萜、皂苷和酚类化合物。提取物的抑制浓度（IC50：24.65）与抗坏血酸相当。标准抗氧化剂的抑制浓度（IC50：20.10）。在酶测定试验中，评估了提取物的 Col-I、Ela-I 和 Hya-I 的抑制浓度，结果显示 Col-I（IC50：21.36）、Ela-I（IC50：35.05）和 Hya-I（IC50：23.44）的抑制浓度分别为 Col-I、Ela-I 和 Hya-I。因此，迷迭香的甲醇提取物可抑制与衰老相关的 Col-I、Ela-I 和 Hya-I 酶活性的 50%。阴性对照（即 UV 处理组）的皱纹评分是 2.83±0.408，而迷迭香甲醇提取物处理组的皱纹评分是 1.83±0.753。