Su Chin-Cheng, Lin Jaung-Geng, Chen Guang-Wei, Lin Wen-Chuan, Chung Jing-Gung
School of Chinese Medicine, China Medical University, Taichung City 404, Taiwan, R.O.C.
Department of Pharmacology, China Medical University, Taichung City 404, Taiwan, R.O.C.
Cancer Genomics Proteomics. 2006 Jan-Feb;3(1):55-61. Epub 2006 Jan 1.
Curcumin (diferuloylmethane) exhibited potent inhibitory activities against proliferation and induced apoptosis in several tumor cell lines. It was recently reported that curcumin induced cell cycle arrest in several human cancer cell lines. However, the exact mechanisms are unclear.
Flow cytometry was used to analyze the cell cycle in human colon cancer colo 205 cells treated with various concentrations of curcumin for 48 h. In order to further understand the mechanism of curcumin-induced G2/M arrest, the checkpoint associated with enzymes of the cell cycle were also investigated by Western blotting methods.
Curcumin induced G2/M arrest in the examined cells and these effects were dose- and time-dependent. Futhermore, curcumin induced Wee1 expression and decreased the Cdc25c, cyclin B1 and CDK1 expressions, resulting in the induction of G2/M cell cycle arrest in the colo 205 cells. The cDNA microarray assay was also employed to confirm gene expressions (mRNA Wee1, Cdc25c, cyclin B1 and CDK1).
The results indicate that curcumin promoted the gene expression of Wee1 and inhibited that of Cdc25c, CDK1 and cyclin B1.
姜黄素（二阿魏酰甲烷）在多种肿瘤细胞系中表现出对增殖的强大抑制活性并诱导细胞凋亡。最近有报道称姜黄素在多种人类癌细胞系中诱导细胞周期停滞。然而，确切机制尚不清楚。
采用流式细胞术分析用不同浓度姜黄素处理48小时的人结肠癌colo 205细胞的细胞周期。为了进一步了解姜黄素诱导G2/M期停滞的机制，还通过蛋白质印迹法研究了与细胞周期酶相关的检查点。
姜黄素在受试细胞中诱导G2/M期停滞，且这些作用呈剂量和时间依赖性。此外,姜黄素诱导Wee1表达并降低Cdc25c、细胞周期蛋白B1和CDK1的表达，从而导致colo 205细胞中G2/M期细胞周期停滞。还采用cDNA微阵列分析来确认基因表达（mRNA Wee1、Cdc25c、细胞周期蛋白B1和CDK1）。
结果表明姜黄素促进Wee1基因表达并抑制Cdc25c、CDK1和细胞周期蛋白B1的基因表达。
