Wang Tuan, Wu Xia, Al Rudaisat Mus'ab, Song Yinjing, Cheng Hao
Department of Dermatology and Venereology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 3 Qingchun Road, Zhejiang, 310016, P.R. China.
J Cancer. 2020 Sep 25;11(22):6704-6715. doi: 10.7150/jca.45176. eCollection 2020.
Our study explored the tumor-suppressive effect of curcumin on cervical cancer cells. Cervical cancer is one of the most common cancers among women worldwide. Acquired resistance to chemotherapeutics and toxicity of such drugs has undermined the effectiveness of cervical cancer treatments. Therefore, the identification of novel chemotherapeutics is key to improving the survival of patients with cervical cancer. Curcumin has been shown to have various bioactivities, including antioxidant and antitumor effects; however, its effect on cervical cancer remains elusive. Here, we used the SiHa human cervical cancer cell line to test various concentrations of curcumin on the proliferation and apoptosis of cervical cancer cells. The involvement of autophagy and reactive oxygen species (ROS) in these effects were also tested by using specific autophagy inhibitors and ROS scavengers. Our results showed that curcumin induced ROS accumulation, apoptosis, autophagy, cell cycle arrest, and cellular senescence accompanied by upregulation of p53 and p21 proteins in SiHa cells.
我们的研究探讨了姜黄素对宫颈癌细胞的肿瘤抑制作用。宫颈癌是全球女性中最常见的癌症之一。对化疗药物的获得性耐药性以及此类药物的毒性削弱了宫颈癌治疗的效果。因此，鉴定新型化疗药物是提高宫颈癌患者生存率的关键。姜黄素已被证明具有多种生物活性，包括抗氧化和抗肿瘤作用；然而，其对宫颈癌的作用仍不明确。在此，我们使用SiHa人宫颈癌细胞系来测试不同浓度的姜黄素对宫颈癌细胞增殖和凋亡的影响。还通过使用特异性自噬抑制剂和活性氧（ROS）清除剂来测试自噬和ROS在这些作用中的参与情况。我们的结果表明，姜黄素在SiHa细胞中诱导ROS积累、凋亡、自噬、细胞周期停滞和细胞衰老，并伴有p53和p21蛋白的上调。
