Department of Dairy, Fat and Cosmetics, University of Chemistry and Technology, Prague, Czech Republic.
Department of Dairy, Fat and Cosmetics, University of Chemistry and Technology, Prague, Czech Republic.
Biochem Biophys Res Commun. 2019 Oct 1;517(4):762-766. doi: 10.1016/j.bbrc.2019.08.001. Epub 2019 Aug 5.
Galactooligosaccharides (GOS) are currently attracting considerable interest as prebiotic substances and can be prepared by transgalactosylation reactions from lactose using β-galactosidase. We applied various combinations of the commercial β-galactosidases, such as Nola Fit 5500, Saphera 2600 L, Maxilact LGI 5000 and Maxilact A4 MG to achieve the highest yield of GOS and reduced lactose content. The combination of the Maxilact LGI 5000 and Nola Fit 5500 resulted in amount of GOS 105 g L with lactose content lower than 5 g L, whilst the combination of the Maxilact A4 MG and Maxilact LGI 5000 enzymes led to an increase in GOS to 141,1 g L and decrease of the lactose content to 46,9 g L. The combination of enzymes produced a higher yield of GOS, reduced the concentration of lactose, eventually, increases the efficiency of galactooligosaccharides purification that could be potentially used in the further investigations.
半乳糖寡糖（GOS）作为一种新型的功能性低聚糖，目前受到广泛关注，可以通过β-半乳糖苷酶对乳糖进行转半乳糖基反应制备得到。本研究采用不同的商业β-半乳糖苷酶组合，如 Nola Fit 5500、Saphera 2600 L、Maxilact LGI 5000 和 Maxilact A4 MG，以获得最高产率的 GOS 和降低乳糖含量。Maxilact LGI 5000 和 Nola Fit 5500 的组合产生了 105 g/L 的 GOS，乳糖含量低于 5 g/L，而 Maxilact A4 MG 和 Maxilact LGI 5000 酶的组合导致 GOS 增加到 141.1 g/L，乳糖含量降低到 46.9 g/L。酶的组合提高了 GOS 的产率，降低了乳糖的浓度，最终提高了半乳糖寡糖的纯化效率，这可能在进一步的研究中得到应用。
