College of Stomatology, Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China.
Stomatological Hospital of Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China.
Dent Mater. 2019 Oct;35(10):1397-1407. doi: 10.1016/j.dental.2019.07.024. Epub 2019 Aug 6.
To fabricate a novel nano-hydroxyapatite mineralized silk fibroin (MSF) scaffold in order to diminish the resorption of alveolar ridge and accelerate new bone formation within tooth sockets. Also, to investigate the biocompatibility and osteogenic ability of the MSF in vitro, and the effect of site preservation of the MSF graft in post-extractive sockets in vivo.
SEM, EDX, FTIR and XRD were used to analyze the mineral crystals deposited on the silk fibroin (SF) surface. Pre-osteoblasts (MC3T3-E1) were seeded on SF and MSF scaffolds. Cell viability, distribution and differentiation were examined using a live-dead assay, histological analysis and Alizarin Red S staining. Furthermore, prepared grafts (SF or MSF scaffold) were implanted into the maxillary right first molar sockets of Sprague Dawley rats for 6 weeks and newly formed bone tissue was analyzed by micro-CT and histological examination.
The SEM, EDX, FTIR and XRD analysis demonstrated that granulate nano-hydroxyapatite (nHA) crystals were uniformly distributed on the SF scaffold. In addition, the MSF hydrophilicity measured by water contact angle and swelling ratio was superior to plain SF scaffold. The effect of nHA inorganic crystals on osteogenic differentiation of MC3T3-E1 cells indicated the MSF scaffolds improved osteogenesis. Furthermore, MSF grafts induced more bone formation and reduced the height of alveolar bone resorption after tooth extraction.
The MSF scaffold partially simulated the structure and composition of natural bone matrix. It induced osteogenic differentiation of MC3T3-E1 cells in vitro, and also promoted new bone regeneration in tooth extraction sockets in vivo, indicating it is a biomaterial with great potential for tooth extraction site preservation.
制备新型纳米羟基磷灰石矿化丝素纤维（MSF）支架，以减少牙槽嵴的吸收并加速牙种植窝内新骨形成。同时，研究 MSF 在体外的生物相容性和成骨能力，以及 MSF 移植物在拔牙窝内的原位保存效果。
SEM、EDX、FTIR 和 XRD 用于分析丝素纤维（SF）表面沉积的矿物晶体。将前成骨细胞（MC3T3-E1）接种到 SF 和 MSF 支架上。通过活/死检测、组织学分析和茜素红 S 染色来检查细胞活力、分布和分化。此外，将制备的移植物（SF 或 MSF 支架）植入 Sprague Dawley 大鼠上颌第一磨牙窝中 6 周，通过 micro-CT 和组织学检查分析新形成的骨组织。
SEM、EDX、FTIR 和 XRD 分析表明，颗粒状纳米羟基磷灰石（nHA）晶体均匀分布在 SF 支架上。此外，通过水接触角和溶胀比测量的 MSF 亲水性优于普通 SF 支架。nHA 无机晶体对 MC3T3-E1 细胞成骨分化的影响表明，MSF 支架促进了成骨。此外，MSF 移植物在拔牙后诱导更多的骨形成并减少牙槽骨吸收的高度。
MSF 支架部分模拟了天然骨基质的结构和组成。它在体外诱导 MC3T3-E1 细胞的成骨分化，并且还促进了牙种植窝内新骨再生，表明它是一种具有巨大潜力的用于牙种植窝保存的生物材料。
