Forget G, Sirois P
Comput Biomed Res. 1983 Apr;16(2):160-8. doi: 10.1016/0010-4809(83)90017-4.
A simple program written in Microsoft BASIC for the analysis of radioimmunoassay data is presented. The program was designed for use by unexperienced operators on a TRS-80 Model III microcomputer. Standard curve data are fitted by linear regression after a logit response/loge dose transformation. Standard errors of individual observations are computed, and confidence intervals are obtained for a critical t of 0.1. Standard observations are eliminated when they lie outside the confidence interval from the calculated response on the fitted curve, and this is accomplished until no datum can be eliminated or until the coefficient of determination reaches an operator defined value. The program then prints the regression parameters and the retained standard values. Unknown sample data can then be entered, and the values are computed relative to the final regression parameters of the standard curve. The values can be modified by operator defined correction/dilution factors. The mean value of the slope for 28 assays is -0.99, while the predicted theoretical slope with a loge transformation is -1. This program finds its greatest use in research laboratories where a number of different RIA are performed and where it is of value to have some control over the computation process.
本文介绍了一个用微软BASIC语言编写的用于分析放射免疫分析数据的简单程序。该程序是为TRS - 80 Model III微型计算机上缺乏经验的操作人员设计的。在进行logit响应/自然对数剂量转换后，通过线性回归拟合标准曲线数据。计算各个观测值的标准误差，并针对临界t值0.1获得置信区间。当标准观测值落在拟合曲线上计算出的响应的置信区间之外时，将其剔除，如此反复进行，直到没有数据可以剔除或者决定系数达到操作人员定义的值。然后程序打印回归参数和保留的标准值。接着可以输入未知样本数据，并根据标准曲线的最终回归参数计算其值。这些值可以通过操作人员定义的校正/稀释因子进行修改。28次测定的斜率平均值为 -0.99，而采用自然对数转换的预测理论斜率为 -1。该程序在进行多种不同放射免疫分析且对计算过程进行一定控制很有价值的研究实验室中最有用武之地。
