Archer A C, Green D F, Buckland E J
South West Regional Blood Transfusion Centre, Southmead, Bristol.
J Clin Pathol. 1988 Aug;41(8):918-9. doi: 10.1136/jcp.41.8.918.
A kinetic assay for alanine transferase (ALT) using microtitre plates and commercially available reagents was developed to retain the advantages conferred by multichannel and semiautomated equipment. The time taken to assay 92 samples was less than 10 minutes, and costs of apparatus and reagents compared favourably with most current methods. Although reactions became non-linear above 120 IU/l-1, higher concentrations were "flagged" and accuracy was satisfactory below this point.
开发了一种使用微量滴定板和市售试剂的丙氨酸转氨酶（ALT）动力学测定法，以保留多通道和半自动设备带来的优势。测定92个样品所需的时间不到10分钟，仪器和试剂成本与大多数现有方法相比具有优势。尽管反应在120 IU/l-1以上变为非线性，但较高浓度会被“标记”，在此浓度以下准确性令人满意。
