Morales-Gamba Ruben Dario, Caldas Jôsie Schwartz, Godoy Leandro, Marcon Jaydione Luiz
Graduate Programme in Aquaculture, Nilton Lins University/National Institute of Amazonian Research, Manaus, Brazil.
Department of Animal Science, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
Zygote. 2019 Aug;27(4):259-261. doi: 10.1017/S096719941900039X. Epub 2019 Aug 9.
This study aimed to describe the morphology and sperm quality of free-living adult males of cururu stingray Potamotrygon wallacei, endemic from the Rio Negro basin, Brazilian Amazon. The sperm was collected in loco from the seminal vesicle region and fixed in buffered saline formaldehyde solution for further evaluation of morphometry, sperm plasma membrane integrity and sperm concentration. The spermatozoa presented a total length of 138.25 ± 1.82 μm with a helical shape and a long head. A high percentage of cells with intact membrane (98 ± 2%) and normal spermatozoa (92 ± 1%) were observed. The cell concentration was 0.34 ± 0.05 × 1010 spermatozoa/ml of semen. These observations are unprecedented for potamotrygonid species and will serve as a basis for future management and conservation strategies.
本研究旨在描述来自巴西亚马逊州内格罗河流域的特有物种——库鲁鲁魟（Potamotrygon wallacei）自由生活成年雄性的形态和精子质量。精子从精囊区域就地采集，并固定在缓冲盐水甲醛溶液中，以进一步评估形态测量、精子质膜完整性和精子浓度。精子全长138.25±1.82μm，呈螺旋状，头部较长。观察到高比例的膜完整细胞（98±2%）和正常精子（92±1%）。细胞浓度为0.34±0.05×10¹⁰个精子/ml精液。这些观察结果对于魟科物种来说是前所未有的，将为未来的管理和保护策略提供依据。
