García-Salinas Pablo, Gallego Victor, Asturiano Juan F
Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 46022 Valencia, Spain.
Associació LAMNA per a l'estudi dels elasmobranquis a la Comunitat Valenciana, Fraules 10, 46020 Valencia, Spain.
Animals (Basel). 2021 Jun 25;11(7):1888. doi: 10.3390/ani11071888.
The superorder Batoidea (rays, skates, and relatives), constitutes one of the most threatened group of vertebrates. Strengthening ex situ conservation programs developed in research centers and public aquaria could be a way of addressing this situation. However, captive breeding programs must be improved to prevent the capture of wild animals and to develop proper in situ reintroduction strategies. Sperm extraction and artificial insemination are two techniques commonly used in other threatened species, which could also be used in rays and the like. However, the different reproductive morphologies present within this group of animals may hamper both processes. Here, we present a comparison of the reproductive anatomies of 11 distinct batoid species, emphasizing the important differences between the species when performing sperm extraction or artificial insemination. Both male and female animals, belonging to the Rajidae, Dasyatidae, Torpedinidae and Myliobatidae families, from the Mediterranean Sea were studied. In addition, we describe the procedure to extract sperm using both cannulation and abdominal massage, either from live or dead batoids Finally, the obtention of motile sperm recovered from the oviducal gland of females is described. These techniques generate a new range of possibilities for the conservation of these threatened species.
鳐总目（鳐、魟及其近亲）是受威胁最严重的脊椎动物群体之一。加强在研究中心和公共水族馆开展的迁地保护项目可能是应对这一情况的一种方式。然而，必须改进圈养繁殖项目，以防止捕获野生动物并制定适当的就地放归策略。精子提取和人工授精是常用于其他受威胁物种的两种技术，也可用于鳐类等动物。然而，这组动物中存在的不同生殖形态可能会妨碍这两个过程。在此，我们对11种不同鳐类物种的生殖解剖结构进行了比较，强调了在进行精子提取或人工授精时物种之间的重要差异。研究了来自地中海的属于鳐科、魟科、电鳐科和鲼科的雄性和雌性动物。此外，我们描述了使用插管法和腹部按摩法从活体或死亡的鳐类动物中提取精子的程序。最后，描述了从雌性输卵管腺中获取活动精子的方法。这些技术为保护这些受威胁物种带来了一系列新的可能性。
