商业草药制剂可改善NK65诱导的小鼠异常。

Commercial herbal preparations ameliorate NK65-induced aberrations in mice.

作者信息

Ogugua Victor N, Okagu Innocent U, Onuh Onyekachi M, Uzoegwu Peter N

机构信息

Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria.

出版信息

J Vector Borne Dis. 2019 Apr-Jun;56(2):146-153. doi: 10.4103/0972-9062.263722.

DOI:10.4103/0972-9062.263722

PMID:31397390

Abstract

BACKGROUND & OBJECTIVES: The alarming failure in malaria treatment using conventional drugs calls for urgent search of alternatives; one of which is to exploit natural products such as plants. This study evaluated the effects of three selected commercial herbal preparations on albino mice infected with Plasmodium berghei NK65, a lethal strain of rodent malaria.

METHODS

This study was conducted in the University of Nigeria, Nsukka between February and September 2017. A total of 30 adult albino mice were randomized into six groups of five mice each. Group 1 served as normal control. Mice in Groups 2-6 were parasitized with P. berghei. Group 2 mice were untreated while mice in Groups 3, 4, 5 and 6 were treated with 20 mg/kg body weight of artesunate; and 5 ml/kg body weight of the seleceted commercial herbal preparations designated as HA, HB and HC, respectively. The percent malaria parasitaemia, haematological parameters, lipid profile, liver function markers, antioxidant status and lipid peroxidation index were evaluated using standard protocol.

RESULTS

It was observed that mice in Group 2 had significantly higher percentage of malaria parasitaemia when compared to mice in parasitized and treated groups. Also, haematological dysfunctions, dyslipidaemia, oxidative stress and hepatotoxicity seen in parasitized and untreated mice were restored in parasitized and artesunate- and herbal preparations-treated mice.

INTERPRETATION & CONCLUSION: Findings from the present study revealed that oxidative stress, characterized by low antioxidant status and high lipid peroxidation, contributes to complications in malaria. The results also indicate that the studied commercial herbal preparations possess good antimalarial and ameliorative effects on malaria-induced haematological, lipid, antioxidant and liver aberrations in mice. The acute toxicity profiles of the commercial herbal preparations suggested that they are tolerable and safe at the doses administered.

摘要

背景与目的

传统药物治疗疟疾的惊人失败促使人们迫切寻找替代方法；其中之一是开发天然产物，如植物。本研究评估了三种选定的商业草药制剂对感染伯氏疟原虫NK65（一种致死性啮齿类疟原虫菌株）的白化小鼠的影响。

方法

本研究于2017年2月至9月在尼日利亚大学恩苏卡分校进行。总共30只成年白化小鼠被随机分为六组，每组五只。第1组作为正常对照。第2 - 6组的小鼠感染伯氏疟原虫。第2组小鼠未接受治疗，而第3、4、5和6组的小鼠分别用20 mg/kg体重的青蒿琥酯以及5 ml/kg体重的选定商业草药制剂（分别命名为HA、HB和HC）进行治疗。使用标准方案评估疟原虫血症百分比、血液学参数、血脂谱、肝功能指标、抗氧化状态和脂质过氧化指数。